지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Objective: The purpose of this study is to investigate the combined effects of ginkgo biloba extract, ginkgolide A and B and aspirin on SK-N-MC, human neuroblastoma cell viability and mRNA expression of growth associated protein43 (GAP43), Microtubule-associated protein 2 (MAP2), B-cell lymphoma2 (Bcl2) and protein53 (p53) gene in hypoxia and reperfusion condition. Methods: SK-N-MC cells were cultured with Dulbecco's Modified Eagle's Medium (DMEM) media in $37^{\circ}C$, 5% $CO_2$ incubator. The cells were cultured for 8 hours in non-glucose media and hypoxic condition and for 12 hours in normal media and $O_2$ concentration. Cell survival rate was measured with Cell Counting Kit-8 (CCK-8) reagent assay. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to estimate mRNA levels of GAP43, MAP2, Bcl2, and p53 genes. Results: The ginkgolide A and B increased viable cell number decreased in hypoxic and reperfused condition. The co-treatment of ginkgolide B with aspirin also increased the number of viable cells, however, there was no additive effect. Although there was no increase of mRNA expression of GAP43, MAP2, and Bcl2 in SK-N-MC cells with individual treatment of ginkgolide A, B or aspirin in hypoxic and reperfused condition, the co-treatment of ginkgolide A or B with aspirin significantly increased GAP43 and Bcl2 mRNA levels. In MAP2, only the co-treatment of ginkgolide A and aspirin showed increasing effect. The mRNA expression of p53 had no change in all treating conditions. Conclusion: This study suggests that the combined treatments of Ginkgo biloba extracts and aspirin increase the regeneration of neuroblastoma cells injured by hypoxia and reperfusion.
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참고문헌 (31)
참고문헌 신청
Ahlemeyer B / 2003 / Neuroprotective effects of Ginkgo biloba extract / Cell Mol Life Sci 60:1779~1792
Araya R / 1998 / Hypoxia induces apoptosis in human neuroblastoma SK-N-MC cells by caspase activation accompanying cytochrome c release from mitochondria / FEBS Lett 439:168~172
Chandrasekaran K / 2003 / Neuroprotective effects of bilobalide, a component of Ginkgo biloba extract (EGb 761) in global brain ischemia and in excitotoxicity- induced neuronal death / Pharmacopsychiatry 36:89~94
Chang LC / 2008 / Patterns of combined prescriptions of aspirin-Ginkgo biloba in Taiwan : a population- based study / J Clin Pharm Ther 33:243~249
Chen JX / 1998 / Protective effects of Ginkgo biloba extract against lysophosphatidylcholine-induced vascular endothelial cell damage / Zhongguo Yao Li Xue Bao 19:359~363
Araya R / 1998 / Hypoxia induces apoptosis in human neuroblastoma SK-N-MC cells by caspase activation accompanying cytochrome c release from mitochondria / FEBS Lett 439:168~172
Chandrasekaran K / 2003 / Neuroprotective effects of bilobalide, a component of Ginkgo biloba extract (EGb 761) in global brain ischemia and in excitotoxicity- induced neuronal death / Pharmacopsychiatry 36:89~94
Chang LC / 2008 / Patterns of combined prescriptions of aspirin-Ginkgo biloba in Taiwan : a population- based study / J Clin Pharm Ther 33:243~249
Chen JX / 1998 / Protective effects of Ginkgo biloba extract against lysophosphatidylcholine-induced vascular endothelial cell damage / Zhongguo Yao Li Xue Bao 19:359~363
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