Different expression of human GFAP promoter-derived GFP in different subsets of astrocytes in the mouse brain

Moon, Young-Hye; Kim, Hyun-Jung; Kim, Joo-Yeon; Kim, Hyun; Kim, Woon-Ryoung; Sun, Woong

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자료유형: 학술저널

저자정보: Moon, Young-Hye (Department of Anatomy, BK21 program, Korea University College of Medicine) Kim, Hyun-Jung (Department of Anatomy, BK21 program, Korea University College of Medicine) Kim, Joo-Yeon (Department of Anatomy, BK21 program, Korea University College of Medicine) Kim, Hyun (Department of Anatomy, BK21 program, Korea University College of Medicine) Kim, Woon-Ryoung (Department of Anatomy, BK21 program, Korea University College of Medicine) Sun, Woong (Department of Anatomy, BK21 program, Korea University College of Medicine)

저널정보: 한국통합생물학회 Animal cells and systems Animal cells and systems 제15권 제4호

발행연도: 2011.1

수록면: 268 - 273 (6page)

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Transgenic mice expressing green fluorescent protein (GFP) under the control of human glial fibrillary acidic protein promoter (hGFAP) have been utilized for in vivo labeling of astrocytes. Although it has been considered that virtually all astrocytes express GFP in this transgenic mouse, we found that different subsets of GFAP-expressing astrocytes express considerably different levels of GFP in the adult brain. Astrocytes in the spinal cord, the molecular layer of thecerebellum, meninges, white matter, corpus callosum and blood vessels exhibited strong GFP, whereas subsets of astrocytes associated with granule cells in the cerebellum and dentate gyrus did not or only marginally exhibited GFP. We also found that a small subset of GFP-expressing cells in the periglomeruli of the olfactory bulb did not express GFAP immunoreactivity. Collectively, these results suggest that human GFAP promoter-derived GFP expression does not faithfully recapitulate the endogenous GFAP expression in mice, suggesting that upstream regulatory mechanisms controlling GFAP transcription are different in different populations of astrocytes, and may reflect the functional diversity of astrocytes.

#astrocyte #GFAP expression #GFP #transgenic mice #granule cell

참고문헌 (48)

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