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논문 기본 정보

자료유형
학술저널
저자정보
Jee, Byung Chul (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) Kim, Jee Hyun (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) Park, Da Hyun (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) Youm, Hyewon (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) Suh, Chang Suk (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) Kim, Seok Hyun (Department of Obstetrics and Gynecology, Seoul National University College of Medicine)
저널정보
대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제39권 제3호
발행연도
2012.1
수록면
107 - 113 (7page)

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Objective: To determine whether animal age impacts in vitro preantral follicle growth. Effects of hCG, stem cell factor (SCF), and/or insulin-like growth factor (IGF) supplementation in growth medium were also investigated. Methods: Intact preantral follicles were mechanically isolated from fresh ovaries of BDF1 mice and cultured in growth medium for 9 to 11 days. Surviving follicles with antrum formation were transferred to maturation medium for 14 to 18 hours. Follicle survival, antrum formation, and retrieval of metaphase II (MII) oocytes were compared among three age categories (4-5, 7-8, and 10-11 week-old). By using 7- to 8-week-old mice, preantral follicles were cultured in growth medium supplemented with hCG (0, 5, or 10 mIU/mL), SCF (50 ng/mL), IGF-1 (50 ng/mL), and SCF+IGF-1. Results: Seven- to eight-week-old mice showed a higher follicle survival and antrum formation and produced more MII oocytes compared to other groups. In the 7- to 8-week-old mice, supplementation of 5 mIU/mL hCG significantly enhanced the antrum formation but the percentage of MII oocytes was similar to that of the control. Supplementation of SCF+IGF-1 did not enhance follicle survival or antrum formation but the percentage of MII oocytes increased modestly (39.1%) than in the control (28.6%, p>0.05, statistically not significant). Conclusion: Seven- to eight-week-old mice showed better outcomes in growth of preantral follicles in vitro than 4- to 5- or 10- to 11-week-old mice. Supplementation of hCG enhanced antrum formation and supplementation of SCF+IGF-1 yielded more mature oocytes; hence, these should be considered in the growth of preantral follicles in vitro.
#Ovarian follicle #Oocyte #Age groups #Stem cell factor #Insulin-like growth factor

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참고문헌 (36)

참고문헌 신청
Demeestere I / 2009 / Orthotopic and heterotopic ovarian tissue transplantation / Hum Reprod Update 15:649~665 google schola Jeruss JS / 2009 / Preservation of fertility in patients with cancer / N Engl J Med 360:902~911 google schola Rodriguez-Wallberg KA / 2010 / Fertility preservation medicine: options for young adults and children with cancer / J Pediatr Hematol Oncol 32:390~396 google schola Telfer EE / 2008 / A two-step serumfree culture system supports development of human oocytes from primordial follicles in the presence of activin / Hum Reprod 23:1151~1158 google schola Cortvrindt R / 1996 / In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepuberal mice in a simplified culture system / Hum Reprod 11:2656~2666 google schola

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