Clinical Significance of Upregulation of mir-196a-5p in Gastric Cancer and Enriched KEGG Pathway Analysis of Target Genes

Li, Hai-Long; Xie, Shou-Pin; Yang, Ya-Li; Cheng, Ying-Xia; Zhang, Ying; Wang, Jing; Wang, Yong; Liu, Da-Long; Chen, Zhao-Feng; Zhou, Yong-Ning; Wu, Hong-Yan

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자료유형: 학술저널

저자정보: Li, Hai-Long (Department of Clinical Testing Teaching and Research, School of Medical Technology, Gansu Traditional Chinese Medical University) Xie, Shou-Pin (First People's Hospital of Lanzhou City) Yang, Ya-Li (Department of Clinical Testing Teaching and Research, School of Medical Technology, Gansu Traditional Chinese Medical University) Cheng, Ying-Xia (Key Laboratory of Traditional Chinese Herbs and Prescription Innovation and Transformation in Gansu Province, Gansu Traditional Chinese Medical University) Zhang, Ying (Department of Dermatology, The First Hospital of Chinese People's Liberation Army) Wang, Jing (Department of Clinical Testing Teaching and Research, School of Medical Technology, Gansu Traditional Chinese Medical University) Wang, Yong (Department of Clinical Testing Teaching and Research, School of Medical Technology, Gansu Traditional Chinese Medical University) Liu, Da-Long (First People's Hospital of Lanzhou City) Chen, Zhao-Feng (Division of Gastroenterology and Hepatology, First Hospital of Lanzhou University) Zhou, Yong-Ning (Division of Gastroenterology and Hepatology, First Hospital of Lanz) Wu, Hong-Yan

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제5호

발행연도: 2015.1

수록면: 1,781 - 1,787 (7page)

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Background: miRNAs are relatively recently discovered cancer biomarkers which have important implications for cancer early diagnosis, treatment and estimation of prognosis. Here we focussed on expression of mir-196a-5p in gastric cancer tissues and cell lines so as to analyse its significance for clinicopathologic characteristics and generate enriched KEGG pathways clustered by target genes for exploring its potential roles as a biomarker in gastric cancer. Materials and Methods: The expression of mir-196a-5p in poorly, moderate and well differentiated gastric cancer cell lines compared with GES-1 was detected by RT-qPCR, and the expression of mir-196a-5p in gastric cancer tissues comparing with adjacent non cancer tissues of 58 cases were also assessed by RT-qPCR. Subsequently, an analysis of clinical significance of mir-196a-5p in gastric cancer and enriched KEGG pathways was executed based on the miRWalk prediction database combined with bioinformatics tools DAVID 6.7 and Mirfocus 3.0. Results: RT-qPCR showed that mir-196a-5p was up-regulated in 6 poorly and moderate differentiated gastric cancer cell lines SGC-7901, MKN-45, MKN-28, MGC-803, BGC-823, HGC-27 compared with GES-1, but down-regulated in the highly differentiated gastric cancer cell line AGS. Clinical data indicated mir-196a-5p to beup-regulated in gastric cancer tissues (47/58). Overexpression of mir-196a-5p was associated with more extensive degree of lymph node metastasis and clinical stage (P < 0.05; x2 test). Enriched KEGG pathway analyses of predicted and validated targets in miRWalk combined with DAVID 6.7 and Mirfocus 3.0 showed that the targeted genes regulated by mir-196a-5p were involved in malignancy associated biology. Conclusions: Overexpression of mir-196a-5p is associated with lymph node metastasis and clinical stage, and enriched KEGG pathway analyses showed that targeted genes regulated by mir-196a-5p may contribute to tumorgenesis, suggesting roles as an oncogenic miRNA biomarker in gastric cancer.

#Gastric cancer #mir-196a-5p #oncogenic gene #KEGG pathway

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Li, Hai-Long

소속기관 Department of Clinical Testing Teaching and Research, School of Medical Technology, Gansu Traditiona