Berberine Hydrochloride Impact on Physiological Processes and Modulation of Twist Levels in Nasopharyngeal Carcinoma CNE-1 Cells

Li, Cai-Hong; Wu, Dong-Fang; Ding, Hang; Zhao, Yang; Zhou, Ke-Yuan; Xu, De-Feng

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자료유형: 학술저널

저자정보: Li, Cai-Hong (Zhongnan Hospital of Wuhan University) Wu, Dong-Fang (Zhongnan Hospital of Wuhan University) Ding, Hang (Institude of Biochemistry and Molecular Biology, Guangdong Medical College) Zhao, Yang (Ear-nose-throat Department of The Second Affiliated Hospital of Guangdong Medical College) Zhou, Ke-Yuan (Institude of Biochemistry and Molecular Biology, Guangdong Medical College) Xu, De-Feng (College of Food Science and Technology, Guangdong Ocean University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제4호

발행연도: 2014.1

수록면: 1,851 - 1,857 (7page)

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Objective: The main purpose of this work was to investigate the effect of berberine hydrochloride (BH) on the proliferation, apoptosis, migration, and invasion of CNE-1 nasopharyngeal carcinoma cells. Our results shed light on the functional components of traditional Chinese herbs for potential use in modern medicine. Methods: The CNE-1 cell line was treated with different concentrations of BH and effects on cell viability and proliferation were evaluated using the Cell Counting Kit-8 (CCK-8) assay. Anti-migratory and anti-invasive actions of BH were investigated using wound healing assays and the Millicell Hanging cell culture insert system, respectively. Expression of the epithelial-mesenchymal transition (EMT)-related gene twist (Twist) was analyzed by real-time PCR and Western blotting. Apoptosis was estimated with an annexin-V fluorescein (FITC) apoptosis detection kit, as well as with reference to levels of activated caspase-3 of CNE-1 cells before and after treatment with BH utilizing fluorescence spectroscopy. Results: BH was capable of reducing proliferation and viability of CNE-1 cells in a dose- and time-dependent manner, also demonstrating anti-migratory and anti-invasive capacities which correlated with reduction in expression of Twist. Finally, BH was able to induce significant amounts of apoptosis in CNE-1 cells, as demonstrated by an increase in the activity of caspase-3 and in annexin-V staining following treatment. Conclusion: BH extracted from rhizoma coptidis demonstrated an ability to block proliferation, induce apoptosis, and impair the migration and invasion of the CNE-1 cell line Considering these properties, our results suggest that BH could be an important compound for consideration in the treatment of nasopharyngeal carcinoma.

#Berberine hydrochloride #invasion #migration #apoptosis #cell proliferation #CNE-1

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