Aberrant Methylation of RASSF2A in Tumors and Plasma of Patients with Epithelial Ovarian Cancer

Wu, Yu; Zhang, Xian; Lin, Li; Ma, Xiao-Ping; Ma, Ying-Chun; Liu, Pei-Shu

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자료유형: 학술저널

저자정보: Wu, Yu (Department of Gynecology and Obstetrics, Qilu Hospital, Shandong University) Zhang, Xian (Department of Gynecology and Obstetrics, Qilu Hospital, Shandong University) Lin, Li (Department of Gynecology and Obstetrics, Qilu Hospital, Shandong University) Ma, Xiao-Ping (Department of Gynecology and Obstetrics, Qilu Hospital, Shandong University) Ma, Ying-Chun (Department of Gynecology and Obstetrics, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University) Liu, Pei-Shu (Department of Gynecology and Obstetrics, Qilu Hospital, Shandong University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제3호

발행연도: 2014.1

수록면: 1,171 - 1,176 (6page)

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Objective: The tumor suppressor gene, Ras-association domain family (RASSF)2A, is inactivated by promoter hypermethylation in many cancers. The current study was performed to evaluate the methylation status of RASSF2A in epithelial ovarian cancer (EOC) tissues and plasma, and correlations with gene expression and clinicopathologic characteristics. Method: We detected methylation of the RASSF2A gene in tissues and corresponding plasma samples from 47 EOC patients and 14 patients with benign ovarian tumors and 10 with normal ovarian tissues. The methylation status was determined by methylation-specific PCR while gene expression of mRNA was examined by RT-PCR. The EOC cell line, SKOV3, was treated with 5-aza-2'-deoxycytidine (5-azadC). Results: RASSF2A mRNA expression was significantly low in EOC tissues. The frequency of aberrant methylation of RASSF2A was 51.1% in EOC tissues and 36.2% in corresponding plasma samples, whereas such hypermethylation was not detected in the benign ovarial tumors and normal ovarian samples. The expression of RASSF2A mRNA was significantly down-regulated or lost in the methylated group compared to the unmethylated group (p<0.05). After treatment with 5-aza-dC, RASSF2A mRNA expression was significantly restored in the Skov3 cell line. Conclusion: Epigenetic inactivation of RASSF2A through aberrant promoter methylation may play an important role in the pathogenesis of EOC. Methylation of the RASSF2A gene in plasma may be a valuable molecular marker for the early detection of EOC.

#Epithelial ovarian cancer #RASSF2A #hypermethylation #plasma

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