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학술저널
저자정보
Song, Joohyun (School of Chemical and Biological Engineering, Seoul National University) Lee, Eunjee A. (School of Chemical and Biological Engineering, Seoul National University) Cha, Seungwoo (School of Chemical and Biological Engineering, Seoul National University) Kim, Insun (School of Chemical and Biological Engineering, Seoul National University) Choi, Yonghoon (School of Chemical and Biological Engineering, Seoul National University) Hwang, Nathaniel S. (School of Chemical and Biological Engineering, Seoul National University)
저널정보
테크노프레스 Biomaterials and biomedical engineering Biomaterials and biomedical engineering 제2권 제1호
발행연도
2015.1
수록면
33 - 45 (13page)

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To engineer tissue-like structures, cells are required to organize themselves into three-dimensional networks that mimic the native tissue micro-architecture. Here, we present agarose-based multi-well platform incorporated with electrical stimulation to build skeletal muscle-like tissues in a facile and highly reproducible fashion. Electrical stimulation of C2C12 cells encapsulated in collagen/matrigel hydrogels facilitated the formation 3D muscle tissues. Consequently, we confirmed the transcriptional upregulations of myogenic related genes in the electrical stimulation group compared to non-stimulated control group in our multi-well 3D culture platform. Given the robust fabrication, engineered muscle tissues in multi-well platform may find their use in high-throughput biological studies drug screenings.

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