지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The genus Cryptomonas is easily recognized by having two flagella, green brownish color, and a swaying behavior. They have relatively simple morphology, and limited diagnostic characters, which present a major difficulty in differentiating between species of the genus. To understand species delineation and phylogenetic relationships among Cryptomonas species, the nuclear-encoded internal transcribed spacer 2 (ITS2), partial large subunit (LSU) and small subunit ribosomal DNA (rDNA), and chloroplast-encoded psbA and LSU rDNA sequences were determined and used for phylogenetic analyses, using Bayesian and maximum likelihood methods. In addition, nuclear-encoded ITS2 sequences were predicted to secondary structures, and were used to determine nine species and four unidentified species from 47 strains. Sequences of helix I, II, and IIIb in ITS2 secondary structure were very useful for the identification of Cryptomonas species. However, the helix IV was the most variable region across species in alignment. The phylogenetic tree showed that fourteen species were monophyletic. However, some strains of C. obovata had chloroplasts with pyrenoid while others were without pyrenoid, which used as a key character in few species. Therefore, classification systems depending solely on morphological characters are inadequate, and require the use of molecular data.
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참고문헌 (39)
참고문헌 신청
Bourelly, P. / 1970 / Les algues d’eau douce: initiation à la systematique. Tome III: Les algues blues et rouges. Les Eugléniens, Peridinienset Cryptomonadines / Editions N. Boubée & Cie :512
Brett, S. J. / 1986 / A comparative study of periplast structure in Cryptomonas cryophila and C. ovata(Cryptophyceae) / Protoplasma 131:23~31
Butcher, R. W. / 1967 / An introductory account of the smaller algae of British coastal waters. Part IV: Cryptophyceae :54
Clay, B. L. / 1999 / A revised classification of the Cryptophyta / Bot. J. Linn. Soc 131:131~151
Deane, J. A. / 2002 / Cryptomonad evolution: nuclear 18S rDNA phylogeny versus cell morphology and pigmentation / J. Phycol 38:1236~1244
Brett, S. J. / 1986 / A comparative study of periplast structure in Cryptomonas cryophila and C. ovata(Cryptophyceae) / Protoplasma 131:23~31
Butcher, R. W. / 1967 / An introductory account of the smaller algae of British coastal waters. Part IV: Cryptophyceae :54
Clay, B. L. / 1999 / A revised classification of the Cryptophyta / Bot. J. Linn. Soc 131:131~151
Deane, J. A. / 2002 / Cryptomonad evolution: nuclear 18S rDNA phylogeny versus cell morphology and pigmentation / J. Phycol 38:1236~1244
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