지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Biological response of cells to variable conditions should affect the expression level of certain genes. Quantification of these changes in target genes needs stable internal controls. Real-time quantitative polymerase chain reaction (PCR) has traditionally used reference or ‘housekeeping’ genes, that are considered to maintain equal expression in different conditions, to evaluate changes in target genes between samples and experimental conditions. Recent studies showed that some housekeeping genes may vary considerably in certain biological samples. This has not been evaluated in red algae. In order to identify the optimal internal controls for real-time PCR, we studied the expression of eleven commonly used housekeeping genes; elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase, β-actin, polyubiquitin, 30S ribosomal gene, 60S ribosomal gene, beta-tubulin, alpha-tubulin, translation initiation factor, ubiquitin-conjugating enzyme, and isocitrate dehydrogenase in different life-history stages of Bostrychia moritziana. Our results suggest that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 30S ribosomal gene, have the most stable gene expression levels between the different life history stages (male, female, carposporophyte, and tetrasporophyte), while the other genes are not satisfactory as internal controls. These results suggest that the combinations of GAPDH and 30S would be useful as internal controls to assess expression level changes in genes that may control different physiological processes in this organism or that may change in different life history stages. These results may also be useful in other red algal systems.
목차
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참고문헌 (20)
참고문헌 신청
Andersen, C. L. / 2004 / Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets / Cancer Res 64:5245~5250
Asamizu, E. / 2003 / Structural analysis of a Lotus japonicus genome. IV. Sequence features and mapping of seventythree TAC clones which cover the 7.5 Mb regions of the genome / DNA Res 10:115~122
Brodie, J. / 2007 / Reconstructing the Tree of Life: Taxonomy and Systematics of Species Rich Taxa / CRC Press :324~334
Chan, P. -L. / 2014 / Evaluation of reference genes for quantitative realtime PCR in oil palm elite planting materials propagated by tissue culture / PLoS One 9:e99774
Hellemans, J. / 2007 / qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data / Genome Biol 8:R19
Asamizu, E. / 2003 / Structural analysis of a Lotus japonicus genome. IV. Sequence features and mapping of seventythree TAC clones which cover the 7.5 Mb regions of the genome / DNA Res 10:115~122
Brodie, J. / 2007 / Reconstructing the Tree of Life: Taxonomy and Systematics of Species Rich Taxa / CRC Press :324~334
Chan, P. -L. / 2014 / Evaluation of reference genes for quantitative realtime PCR in oil palm elite planting materials propagated by tissue culture / PLoS One 9:e99774
Hellemans, J. / 2007 / qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data / Genome Biol 8:R19
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