지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
This review provides a comprehensive summary of the PCR primers and profiles currently in use in our laboratory for red algal DNA barcoding and phylogenetic research. While work focuses on florideophyte taxa, many of the markers have been applied successfully to the Bangiales, as well as other lineages previously assigned to the Bangiophyceae sensu lato. All of the primers currently in use with their respective amplification profiles and strategies are provided, which can include full fragment, overlapping fragments and what might best be called "informed overlapping fragments", i.e., a fragment for a marker is amplified and sequenced for a taxon and those sequence data are then used to identify the best primers to amplify the remaining fragment(s) for that marker. We extend this strategy for the more variable markers with sequence from the external PCR primers used to "inform" the selection of internal sequencing primers. This summary will hopefully serve as a useful resource to systematists in the red algal community.
목차
등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
Bird, C. J. / 1992 / Phylogenetic relationships in the Gracilariales(Rhodophyta)as determined by 18S rDNA sequences / Phycologia 31:510~522
Clarkston, B. E. / 2010 / A comparison of two DNA barcode markers for species discrimination in the red algal family Kallymeniaceae(Gigartinales, Florideophyceae), with a description of Euthora timburtonii sp. nov / Botany 88:119~131
Clayden, S. L. / 2010 / Recognition of Rubrointrusa membranacea gen. et comb. nov., Rhodonematella subimmersa gen. et comb. nov. (with a reinterpretation of the life history)and the Meiodiscaceae fam. nov. within the Palmariales(Rhodophyta) / Phycologia 49:283~300
Druehl, L. D. / 2005 / An evaluation of methods used to assess intergeneric hybridization in kelp using Pacific Laminariales (Phaeophyceae) / J. Phycol 41:250~262
Freshwater, D. W. / 1998 / A multigene phylogeny of the Gelidiales including nuclear large-subunit rRNA sequence data / J. Appl. Phycol 10:229~236
Clarkston, B. E. / 2010 / A comparison of two DNA barcode markers for species discrimination in the red algal family Kallymeniaceae(Gigartinales, Florideophyceae), with a description of Euthora timburtonii sp. nov / Botany 88:119~131
Clayden, S. L. / 2010 / Recognition of Rubrointrusa membranacea gen. et comb. nov., Rhodonematella subimmersa gen. et comb. nov. (with a reinterpretation of the life history)and the Meiodiscaceae fam. nov. within the Palmariales(Rhodophyta) / Phycologia 49:283~300
Druehl, L. D. / 2005 / An evaluation of methods used to assess intergeneric hybridization in kelp using Pacific Laminariales (Phaeophyceae) / J. Phycol 41:250~262
Freshwater, D. W. / 1998 / A multigene phylogeny of the Gelidiales including nuclear large-subunit rRNA sequence data / J. Appl. Phycol 10:229~236
이 논문의 저자 정보
최근 본 자료
댓글(0)
0