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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Yong-Kyoung (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University) Yang, Tae-Jin (Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University) Kim, Soo-Un (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University) Park, Sang-Un (Department of Crop Science, Chungnam National University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제55권 제1호
발행연도
2012.1
수록면
27 - 34 (8page)

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Panax ginseng produces a large number of ginsenosides throughout the parts of the ginseng plant. Several genes in the ginsenoside biosynthesis pathway were cloned, including those encoding farnesyl diphosphate synthase, squalene synthase, squalene epoxidase, dammarenediol-II synthase, lanosterol synthase, ${\beta}$-amyrin synthase, and cycloartenol synthase. The expression levels of these seven genes were examined in the roots and different developmental stages of the flowers and berries of 4-year-old ginseng plants using quantitative real-time polymerase chain reaction. The levels of ten ginsenosides ($Rb_1$, $Rb_2$, $Rb_3$, Rc, Rd, Re, Rf, $Rg_1$, $Rg_2$, and $Rh_1$) were determined by high performance liquid chromatography analysis. Gene expression varied during flower and berry development. Total saponin levels were highest in the early berry stage and correlated well with those in the ginseng root. Therefore, berries should be harvested at the early berry stage to ensure optimal ginsenoside levels for pharmaceutical interest.

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