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논문 기본 정보

자료유형
학술저널
저자정보
Park, Sun-Hwa (School of Environmental Science and Engineering, Gwangju Institute of Science and Technology) Ryu, Ji-Young (Marine Biotechnology Research Center, Korea Ocean Research & Development Institute) Seo, Ji-Young (School of Environmental Science and Engineering, Gwangju Institute of Science and Technology) Hur, Hor-Gil (School of Environmental Science and Engineering, Gwangju Institute of Science and Technology)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제55권 제1호
발행연도
2012.1
수록면
83 - 88 (6page)

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초록· 키워드

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Indigo dye has been used in the textile dye industry for long period. Insoluble indigo are reduced to soluble leuco-indigo before dying textiles. A traditional process for solubilization of indigo using microbial reduction metabolism has been considered as environmentally benign method and as alternative to faster chemical reactions. Thus, fermentation liquor aged for 6 years with Polygonum tinctorium (indigo plant) extracts was used to isolate bacteria able to reduce insoluble indigo. Two bacterial isolates, A1 and G5, showed indigo-reducing activity, and were identified as Alkalibacterium sp. and Pseudomonas sp. respectively, with 99% sequence similarity by 16S rDNA sequence analyses. Based on the concentrations of leuco-indigo reduced from indigo, Alkalibacterium sp. A1 and Pseudomonas sp. G5 showed alkaliphilic and thermotolerant charactertistics, optimally functioning at pH 10.0 and $50^{\circ}C$. Isolation of alkaliphilic and thermotolerant bacterial strains, which can reduce insoluble indigo into leuco-indigo, from Korean traditional fermentation liquor could provide a biological tool to enhance efficiency in the traditional indigo dye by an environmentally friendly manner.

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