Expression Study of a Recombinant Plasmid containing Dipeptidyl Peptidase-4 Gene in E. coli: A Plausible Application for Celiac Disease Patients to Digest Gluten

Lee, Yeonjae; Kang, Ryan; Kwon, Jenna; Jo, Kyuhee; Im, Jungbin; Jung, Sangwook; Lee, DongHyun; Lee, Juhyeon; Lee, Jeong-Sang

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자료유형: 학술저널

저자정보: Lee, Yeonjae (Hankuk Academy of Foreign Studies) Kang, Ryan (Hankuk Academy of Foreign Studies) Kwon, Jenna (Hankuk Academy of Foreign Studies) Jo, Kyuhee (Hankuk Academy of Foreign Studies) Im, Jungbin (Hankuk Academy of Foreign Studies) Jung, Sangwook (Hankuk Academy of Foreign Studies) Lee, DongHyun (Hankuk Academy of Foreign Studies) Lee, Juhyeon (Hankuk Academy of Foreign Studies) Lee, Jeong-Sang (Dep. of Biotechnology and Functional Foods, Jeonju University)

저널정보: 한국인터넷방송통신학회 International journal of advanced smart convergence International journal of advanced smart convergence 제7권 제2호

발행연도: 2018.1

수록면: 101 - 111 (11page)

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Celiac disease (CD) is an immune-mediated enteropathy of small intestine diagnosed in both childhood and adulthood. CD is caused by gluten, which produces gliadorphin during its digestion. The enzyme dipeptidyl peptidase-4 (DPP4) breaks gliadorphin down nevertheless the last tripeptide remains and eventually inhibits DPP4, thus slowing down its process. Therefore, the idea is to produce an additional DPP4 enzyme which is crucial. Consequently, the functional DPP4 gene was cloned into pCDNA3 intermediate (FLAG+DPP4) vector and finally a recombinant plasmid pSB1C3 (Andersons promoters+FLAG+DPP4) was constructed using synthetic biology. Normally, a DPP4 inhibitor is used as a cure for diabetes. Another important concern was overexpression of DPP4, which might lead to diabetes, accordingly the work was also performed for the regulation of the DPP4 gene expression. In this regard, three types of Anderson promoters (strong, moderate and weak) were utilized to study the control overexpression. This is the first report of idealistic trial for control the exogenous DPP4 gene-expression by molecular biologic tools.

#Celiac disease #Vectors #Anderson promoter #Infusion ligation #Synthetic biology

참고문헌 (15)

참고문헌 신청

J.M. Turner / 2018 / Diagnosis of Celiac Disease: Taking a Bite Out of the Controversy / Digestive Disease and Sciences (DDS) 63(6):1384~1391

J.T. Rosenbaum / 2017 / Celiac Disease and Autoimmunity-The Missing Ingredient / New England Journal of Medicine 377(15):1489~1490

J. West / 2014 / Incidence and Prevalence of Celiac Disease and Dermatitis Herpetiformis in the UK Over Two Decades: Population-Based Study / The American Journal Of Gastroenterology(AJG) 109:757~768

E. Almallouhi / 2017 / Increasing Incidence and Altered Presentation in a Population-based Study of Pediatric Celiac Disease in North America / Journal of Pediatric Gastroenterology and Nutrition 65(4):432~437

M.M. Leonard / 2017 / Celiac Disease and Nonceliac Gluten Sensitivity-a Review / JAMA 318(7):647~656

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