Individual Identification using The Multiplex PCR with Microsatellite Markers in Swine

Kim, Lee-Kung; Park, Chang-Min; Park, Sun-Ae; Kim, Seung-Chang; Chung, Hoyoung; Chai, Han-Ha; Jeong, Gyeong-Yong; Choi, Bong-Hwan

추천

검색

자료유형: 학술저널

저자정보: Kim, Lee-Kung (Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA) Park, Chang-Min (Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA) Park, Sun-Ae (Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA) Kim, Seung-Chang (Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA) Chung, Hoyoung (Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA) Chai, Han-Ha (Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA) Jeong, Gyeong-Yong (Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA) Choi, Bong-Hwan (Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA)

저널정보: 한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제37권 제4호

발행연도: 2013.1

수록면: 205 - 211 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

The swine is one of the most widespread mammalian throughout the whole world. Presently, many studies concerning microsatellites in swine, especially domestic pigs, have been carried out in order to investigate general diversity patterns among either populations or breeds. Until now, a lot of time and effort spend into a single PCR method. But simple and more rapid multiplex PCR methods have been developed. The purpose of this study is to develop a robust set of microsatellites markers (MS marker) for traceability and individual identification. Using multiplex-PCR method with 23 MS marker divided 2 set, various alleles occurring to 5 swine breed (Berkshire, Landrace, Yorkshire, Duroc and Korea native pig) used markers to determine allele frequency and heterozygosity. MS marker found 4 alleles at SW403, S0227, SWR414, SW1041 and SW1377. The most were found 10 alleles at SW1920. Heterozygosity represented the lowest value of 0.102 at SWR414 and highest value of 0.861 at SW1920. So, it was recognized appropriate allele frequency for individual identification in swine. Using multiplex-PCR method, MS markers used to determine individual identification biomarker and breed-specific marker for faster, more accurate and lower analysis cost. Based on this result, a scientific basis was established to the existing pedigree data by applying genetics additionally. Swine traceability is expected to be very useful system and be conducted nationwide in future.

#Microsatellite marker #Swine #Multiplex PCR #Individual identification #traceability

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요