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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Hyun (Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo) Matsuwaki, Takashi (Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo) Yamanouchi, Keitaro (Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo) Nishihara, Masugi (Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo) Kim, Sung-Woo (Animal Genetic Resources Station, National Institute of Animal Science, RDA) Ko, Yeoung-Gyu (Animal Genetic Resources Station, National Institute of Animal Science, RDA) Yang, Boh-Suk (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제35권 제3호
발행연도
2011.1
수록면
341 - 348 (8page)

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Sloan-Kettering virus gene product of a cellular protooncogene c-Ski is an unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. The aim of the present study was to locate Ski protein in rat ovaries in order to predict the possible involvement of Ski in follicular development and atresia. First, expression of c-Ski mRNA in the ovaries of adult female rats was confirmed by RT-PCR. Then, ovaries obtained on the day of estrus were subjected to immunohistochemical analysis for Ski and proliferating cell nuclear antigen (PCNA) in combination with terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). Ski was expressed in granulosa cells that were positive for TUNEL, but negative for PCNA, regardless of the shape and size of follicles. Expression of Ski in TUNEL-positive granulosa cells, but not in PCNA-positive granulosa cells, was also verified in immature hypophysectomized rats having a single generation of developing and atretic follicles by treatment with equine chorionic gonadotropin (eCG). These results indicate that Ski is profoundly expressed in the granulosa cells of atretic follicles, but not in growing follicles, and suggest that Ski plays a role in apoptosis of granulosa cells during follicular atresia.

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