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논문 기본 정보

자료유형
학술저널
저자정보
Kwon, Dae-Jin (Animal Biotechnology Division, National Institute of Animal Science, RDA) Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA) Ock, Sun A (Animal Biotechnology Division, National Institute of Animal Science, RDA) Lee, Jeong Woong (Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) Lee, Sung-Soo (Animal Biotechnology Division, National Institute of Animal Science, RDA) Park, Jin-Ki (Animal Biotechnology Division, National Institute of Animal Science, RDA) Chang, Won-Kyong (Animal Biotechnology Division, National Institute of Animal Science, RDA) Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제36권 제4호
발행연도
2012.1
수록면
249 - 254 (6page)

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초록· 키워드

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This study aimed at investigating whether a porcine follicular fluid (pFF) supplementation positively affects the characteristics of donor cells and the developmental competence of porcine cloned embryos. Ear fibroblast cells (donor cell) from an Massachusetts General Hospital miniature pig were cultured in different culture methods: (1) Dulbecco's modified Eagle's medium (DMEM)+10% FBS (Control); (2) DMEM+0.5% FBS (SS); and (3) DMEM+10% FBS+10% pFF (pFF) for 72 h. In each conditioned medium, the concentrations of 4 amino acids (Thr, Glu, Pro, and Val) in the pFF group were significantly different from those in the control group (p<0.05 or p<0.01). The proliferation of the cells cultured in the SS group was significantly lower than that of the other treatment groups (p<0.01). The population of apoptotic and necrotic cells in the SS group was significantly higher than that of either the control or the pFF group (p<0.01). The number of embryos that cleaved (p<0.05) and developed into blastocysts (p<0.01) in the SS group was significantly lower than that of either the control or the pFF group. Compared to other groups, the blastocysts produced from the donor cells in the pFF group had higher total cells and lower apoptotic cells (p<0.05). It can be concluded that pFF supplementation in the donor cell culture medium positively affects cell death, cell cycle and quality of the cloned blastocyst.

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