Effects of Roscovitine on In Vitro Development of Porcine Oocyte Using Brilliant Cresyl Blue

Roy, Pantu Kumar; Fang, Xun; Hassan, Bahia MS; Shin, Sang Tae; Cho, Jong Ki

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자료유형: 학술저널

저자정보: Roy, Pantu Kumar (College of Veterinary Medicine, Chungnam National University) Fang, Xun (College of Veterinary Medicine, Chungnam National University) Hassan, Bahia MS (College of Veterinary Medicine, Chungnam National University) Shin, Sang Tae (College of Veterinary Medicine, Chungnam National University) Cho, Jong Ki (College of Veterinary Medicine, Chungnam National University)

저널정보: 한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제32권 제3호

발행연도: 2017.1

수록면: 111 - 122 (12page)

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The objective of this experiment was to explore the effects of Roscovitine (Rosco) prior to in vitro maturation (IVM) of immature pig oocyte. Brilliant cresyl blue test has been used to select the good quality of oocyte. Specifically, the effects of Rosco exposure on nuclear and cytoplasmic maturation, diameter, intracellular glutathione (GSH) and reactive oxygen species (ROS), and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT), and gene expression levels in SCNT embryos have been measured. Cumulus oocyte complexes (COCs) have been exposed in $75{\mu}M$ of Rosco for 22 and 44 h. The COCs that were matured in the IVM for 44 h without Rosco used as control group. Diameter of matured porcine oocytes 44 h culture with Rosco was significantly lower than 22 h culture with Rosco and control groups. GSH was higher in control group than 22 h and 44 h with Rosco but reduction of ROS in 22 h than 44 h with Rosco. In PA, exposure with Rosco 44 h oocytes group has been significantly lower than 22 h and control group in rates of maturation, cleavage and blastocyst formation. Similarly, in SCNT embryos rates of maturation, cleavage and formation of blastocyst have been also significantly lower in 44 h Rosco treated group than other two groups. SCNT embryos treated with Rosco 22 h showed greater expression levels of POU5F1, DPPA2 and NDP52Il mRNA compared with other two groups. Our results demonstrate that Rosco treatment with 22 h prior to IVM improves the development competence of porcine oocyte.

#Brilliant cresyl blue #Cloning #embryo #Parthenogenesis #Roscovitine

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