지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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The purpose of this study was to isolate bacteriocin-producing bacteria with antagonistic activities against pathogens from the intestines of pigs for probiotic use. Lactobacillus sp. AP 116 possessing antimicrobial property was selected from a total of 500 isolates. The AP 116 strain showed a relatively broad spectrum of inhibitory activity against Listeria monocytogenes, Clostridium perfringens, Pediococcus dextrinicus, and Enterococcus strains using the spot-on-lawn method. Bacteriocin activity remained unchanged after 15 min of heat treatment at $121^{\circ}C$ and exposure to organic solvents; however, it diminished after treatment with proteolytic enzymes. Maximum production of bacteriocin occurred at $34^{\circ}C$ when a pH of 6.0 was maintained throughout the culture during fermentation. According to a tricine SDS-PAGE analysis, the molecular weight of the bacteriocin was approximately 5 kDa. The isolate tolerated bile salts and low pH, and also induced nitric oxide (NO) in mouse peritoneal macrophages. Bacteriocin and bacteriocin-producing bacteria, such as Lactobacillus sp. AP 116, could be potential candidates for use as probiotics as an alternative to antibiotics in the pig industry.
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참고문헌 (34)
참고문헌 신청
Aasen, I. M. / 2000 / Influence of complex nutrients, temperature and pH on bacteriocin production by Lactobacillus sakei CCUG 42678 / Applied Microbiology and Biotechnology 53:159~166
Bhunia, A. K. / 1991 / Mode of action of pediocin AcH from Pediococcus acidilactici H on sensitive bacterial strains / Journal of Applied Bacteriology 70:25~33
Byun, J. W. / 2000 / In vitro selection of lactic acid bacteria for probiotic use in pigs / Korean Journal of Veterinary Research 40:701~706
Callaway, T. R. / 2004 / Recent pre-harvest supplementation strategies to reduce carriage and shedding of zoonotic enteric bacterial pathogens in food animals / Animal Health Research Reviews 5:35~47
Cleveland, J. / 2001 / Bacteriocins: safe, natural antimicrobials for food preservation / International Journal of Food Microbiology 71:1~20
Bhunia, A. K. / 1991 / Mode of action of pediocin AcH from Pediococcus acidilactici H on sensitive bacterial strains / Journal of Applied Bacteriology 70:25~33
Byun, J. W. / 2000 / In vitro selection of lactic acid bacteria for probiotic use in pigs / Korean Journal of Veterinary Research 40:701~706
Callaway, T. R. / 2004 / Recent pre-harvest supplementation strategies to reduce carriage and shedding of zoonotic enteric bacterial pathogens in food animals / Animal Health Research Reviews 5:35~47
Cleveland, J. / 2001 / Bacteriocins: safe, natural antimicrobials for food preservation / International Journal of Food Microbiology 71:1~20
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