지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 665 - 673 (9page)
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초록· 키워드
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We compared standard culture methods as well as conventional PCR and real-time PCR for the detection of Listeria monocytogenes (L. monocytogenes) in milk, cheese, fresh-cut vegetables, and raw beef that have different levels of background microflora. No statistical differences were observed in sensitivity between the two selective media in all foods. In total, real-time PCR assay exhibited statistically excellent detection sensitivity (p<0.05) and was less time consuming and laborious as compared with standard culture methods. Conventional culture methods showed poor performance in detecting L. monocytogenes in food with high levels of background microflora, generating numerous false negative results. While the detection of L. monocytogenes in fresh cut vegetable by culture methods was hindered only by L. innocua, various background microflora, such as L. innocua, L. welshimeri, L. grayi, and Enterococcus faecalis appeared on the two selective media as presumptive positive colonies in raw beef indicating the necessity of improvement of current selective media. It appears that real-time PCR is an effective and sensitive presumptive screening tool for L. monocytogenes in various types of foods, especially foods samples with high levels of background microflora, thus complementing standard culture methodologies.
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참고문헌 (39)
참고문헌 신청
Almeida, P. F / 2000 / A PCR protocol using inl gene as a target for specific detection of Listeria monocytogenes / Food Control 11:97~101
Amagliani, G / 2007 / Detection of Listeria monocytogenes using a commercial PCR kit and different DNA extraction methods / Food Control 18:1137~1142
Amagliani, G / 2006 / Development of a magnetic capture hybridization-PCR assay for Listeria monocytogenes direct detection in milk samples / J. Appl. Microbiol 100:375~383
Berrada, H / 2006 / Quantification of Listeria monocytogenes in salads by real time quantitative PCR / Int. J. Food Microbiol 107:202~206
천정환 / 2012 / Development of Real-Time PCR for the Detection of Clostridium perfringens in Meats and Vegetables / Journal of Microbiology and Biotechnology 22(4):530~534
Amagliani, G / 2007 / Detection of Listeria monocytogenes using a commercial PCR kit and different DNA extraction methods / Food Control 18:1137~1142
Amagliani, G / 2006 / Development of a magnetic capture hybridization-PCR assay for Listeria monocytogenes direct detection in milk samples / J. Appl. Microbiol 100:375~383
Berrada, H / 2006 / Quantification of Listeria monocytogenes in salads by real time quantitative PCR / Int. J. Food Microbiol 107:202~206
천정환 / 2012 / Development of Real-Time PCR for the Detection of Clostridium perfringens in Meats and Vegetables / Journal of Microbiology and Biotechnology 22(4):530~534
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