지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 281 - 286 (6page)
이용수
초록· 키워드
오류제보하기
This study was to evaluate the protein profile of seminal plasma using 2-DE in Hanwoo. Seminal plasma was harvested from five mature Hanwoo, and seminal plasma protein was extracted by M-PER Mammalian Protein Extraction Reagent. Proteins were refined by clean-up kit and quantified by Bradford method until total protein was $300\;{\mu}l$. Immobilized pH gradient (IPG) strip was used 18 cm and 3~11 NL. SDS-PAGE was used 12% acrylamide gel. Each gels were visualized by comassie brilliant blue and silver staining. These spots were analyzed by MALDI-TOF MS and searched on NCBInr. The result, 20 proteins of 36 protein spots were searched through peptide sequencing on the NCBInr. 8 proteins profiled by 2-DE were proved through previous bovine studies and the name of each protein was albumin, nucleobindin, clusterin, TIMP-2, spermadhesin Z13, spermadhesin-1 and BSP proteins (BSP 30 kDa and BSP A1/A2). 12 new proteins were ATP synthase, protein MAK16 homolog, Transmembrane protein 214, E3 ubiquitin-protein ligase BRE1A, dual serine/threonine and tyrosine protein kinase, tissue factor pathway inhibitor 2, alpha-actinin-4, RUN domain-containing protein 3B, catenin alpha-1, protein-glutamine gamma-glutamyltransferase 2, plakophilin-1 and inter-alpha-trypsin inhibitor heavy chain H1 has not been previously described in the bovine seminal plasma study. These proteins may be contribute to define the type of proteins affecting fertility of male and improve the fertilizing ability of semen in Hanwoo.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Analysis of Bovine Seminal Plasma Proteins from Korean Native Cattle, Hanwoo, and Korean Native Brindle Cattle
한국동물번식학회지
2012 .01
-
Analysis of Hanwoo Loin Proteome by 2-D Gel Electrophoresis and Peptide Mass Fingerprinting
Journal of Food Science and Nutrition
2002 .12
-
돼지 난포 발달 시 과립막 세포에서 발현되는 단백질의 변화
한국동물번식학회지
2009 .01
-
A Proteome Reference Map for Porcine Plasma Proteins
한국동물번식학회지
2013 .01
-
Identification of Bovine Pregnancy-Specific Whey Proteins using Two-Dimensional Gel Electrophoresis
한국동물번식학회지
2008 .01
-
쌀단백질의 가치와 이용방법
식품과학과 산업
2019 .03
-
2-DE and MALDI-TOF MS-based identification of bovine whey proteins in milk collected soon after parturition
Korean Journal of Agricultural Science
2018 .12
-
Development of Methods for Protein Extraction from Three Major Korean Fermented Soy Foods for 2-Dimensional Gel and Mass Spectrometric Analyses
Journal of applied biological chemistry
2008 .01
-
Protein Expression of Mouse Uterus in Post-Implantation
한국동물번식학회지
2009 .01
-
[P3-18] Recovery of Muscle Protein from Large Anchovy and Gel Properties of Recovered Protein
한국식품영양과학회 학술대회발표집
2006 .10
-
Effect of Gamma - Irradiation on the Molecular Properties of Blood Plasma Proteins
Journal of Food Science and Nutrition
2002 .06
-
은어 사료의 적정 단백질 함량
한국양식학회지
1999 .06
-
Profiling of differentially expressed proteins in mature kernels of Korean waxy corn cultivars using proteomic analysis
Applied Biological Chemistry
2015 .01
-
적소두단백질(Red Bean Protein)과 Transglutaminase를 첨가한 돈육 근원섬유 단백질의 물성 증진 효과
한국축산식품학회지
2011 .01
-
Inheritance of P34 Allergen Protein in Mature Soybean Seed
한국육종학회지
2011 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0