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자료유형
학술저널
저자정보
Park, Min Hee (Department of Animal Life Science, Kangwon National University) Park, Ji Eun (Department of Animal Life Science, Kangwon National University) Kim, Min Seong (Department of Animal Life Science, Kangwon National University) Lee, Kwon Young (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Yun, Jung Im (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Choi, Jung Hoon (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Lee, Eunsong (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Lee, Seung Tae (Department of Animal Life Science, Kangwon National University)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제29권 제3호
발행연도
2014.1
수록면
221 - 228 (8page)

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Despite that porcine spermatogonial stem cells (pSSCs) have been regarded as a practical tool for preserving eternally genetic backgrounds derived from pigs with high performance in the economic traits or phenotypes of specific human diseases, there were no reports about precise definition of niche conditions promoting proliferation and maintenance of pSSCs. Accordingly, we tried to determine niche conditions supporting proliferation and maintenance of undifferentiated pSSCs for short-term. For these, undifferentiated pSSCs were progressively cultured in different composition of culture medium, seeding density of pSSCs, type of feeder cells and concentration of growth factors, and then total number of and alkaline phosphatase (AP) activity of pSSCs were investigated at post-6 day culture. As the results, the culture of $4{\times}10^5$ pSSCs on mitotically in activated $2{\times}10^5$ STO cells in the mouse embryonic stem cell culture medium (mESCCM) supplemented with 30 ng/ml glial cell line-derived neurotrophic factor (GDNF) was identified as the best niche condition supporting effectively the short-term maintenance of undifferentiated pSSCs. Moreover, the optimized short-term culture system will be a basis for developing long-term culture system of pSSCs in the following researches.

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