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논문 기본 정보

자료유형
학술저널
저자정보
Jung, Hyun-Ju (Animal Biotechnology Division, National Institute of Animal Science, RDA) Ko, Yeoung-Gyu (Animal Biotechnology Division, National Institute of Animal Science, RDA) Hwang, Seong-Soo (Animal Biotechnology Division, National Institute of Animal Science, RDA) Im, Gi-Sun (Animal Biotechnology Division, National Institute of Animal Science, RDA) Park, Mi-Rung (Animal Biotechnology Division, National Institute of Animal Science, RDA) Woo, Jae-Seok (Animal Biotechnology Division, National Institute of Animal Science, RDA) Park, Choon-Keun (College of Animal Life Science, Kangwon National University) Seong, Hwan-Hoo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제31권 제4호
발행연도
2007.1
수록면
227 - 233 (7page)

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This study was conducted to examine the mRNA expression of apoptosis-related and imprinted genes and methylation pattern of the differentially methylated region (DMR) of H19 gene in day 35 of SCNT pig fetuses. The day 35 of natural mating (control) or cloned (clone) pig fetuses were recovered from uterus. Endometrium from dam and liver from fetus were obtained, respectively. mRNA expression was evaluated by real-time PCR and methylation pattern was analyzed by bisulfite sequencing method. The Bcl-2 mRNA expression in clone was significantly lower than that of control (p<0.05). The mRNA expression of H19 gene in both endometrium and liver was significantly higher in clone than that of control, respectively (p<0.05). The level of IGF-2 mRNA in liver of clone was significantly lower than that of control (p<0.05), whereas the mRNA expression of IGF2-R gene in liver of clone was significantly higher than that of control (p<0.05). The DMR of H19 was lower methylation pattern in clone than that of control. These results suggest that the aberrant mRNA expression of apoptosis-related and imprinted genes and the lower DMR methylation pattern of imprinted gene may be closely related to the inadequate fetal development of cloned fetus.

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