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논문 기본 정보

자료유형
학술저널
저자정보
Jeon, Gi-Jun (National livestock Research Institute, RDA) Jang, Hyun-Yong (College of Animal Life Science Kangwon National University) Cheong, Hee-Tae (School of Veterinary Medicine Kangwon National University) Kim, Jong-Taek (School of Veterinary Medicine Kangwon National University) Park, Choon-Keun (College of Animal Life Science Kangwon National University) Chu, Duong-Xuan (College of Pharmacy, Kangwon National University) Lee, Hak-Kyu (Genomic Informatics Center, Hankyong National University) Yang, Boo-Keun (College of Animal Life Science Kangwon National University)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제31권 제1호
발행연도
2007.1
수록면
49 - 53 (5page)

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Astaxanthin is a kind of carotenoid compounds, having a antioxidant and anti-inflammatory activities. The antioxidative mechanism by which carotenoid scavenge free radicals has been clearly elucidated, but has not tried for the development of mammalian preimplantation embryo. This study was conducted to investigate the antioxidative effect of astaxanthin on in vitro development of porcine in vitro fertilized embryos. Porcine embryos derrived from in vitro fertilization (IVF) were cultured in 5% $CO_{2} in air at $38.5^{\circ}C$ in PZM-3 medium supplemented with different dosages of astaxanthin ($0,\;1,\;5\;and\;10{\mu}M$) and taurine (0, 1, 2.5 and 5 mM) as a positive control, and execute to compare the effects of various antioxidants such as taurine, melatonin and asculatin on in vitro development. The proportions of embryos developed to the blastocyst stage were increased when $1\;and\;5\;{\mu}M$ of astaxanthin (26.6 and 23.4%, respectively) and 1 and 2.5 mM taurine (25.8 and 26.4%, Respectively) were supplemented, compared to controls (p<0.05). Also, various antioxidant-treated groups were significantly higher rates of blastocysts (astaxanthin, 27.4%; taurine, 29.1%; melatonin, 26.8%; aesculetin, 27.9%, respectively) than control (18.8%). There was no difference in mean cell number of blastocysts between antioxidants and control. This result indicates that astaxanthin has an antioxidant feature when porcine IVF embryos were cultured in vitro.

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