지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
We performed Agrobacterium-mediated genetic transformation of creeping bentgrass (Agrostis stolonifera L.) and produced herbicide-resistant transformants from commercial cultivars Crenshaw and Penncross. Seed-derived embryogenic calli were infected with A. tumefaciens EHA105 harboring pCAMBIA 3301, which includes an intron-containing gus reporter and a bar selection marker. To establish a stable system, we examined various factors that could potentially influence transformation efficiency during the pre-culture, infection, and co-cultivation steps. The addition of kinetin to the callus pre-culture media increased efficiency about three-fold. Once the optimum infection and co-cultivation conditions were identified, this protocol was used successfully to bulk-produce herbicide-resistant transgenic plants whose herbicide resistance was confirmed using the $BASTA^{(R)}$ resistance test. Southern blot analysis demonstrated integration and low copy numbers of the integrated transgenes, and northern blot analysis verified their expression. Thus, we have established an efficient genetic transformation system for creeping bentgrass and confirmed a high frequency of single-copy transgene integration and functional gene expression.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
NaOH처리에 의한 Agrobacterium이용 팽이균사체 형질전환
한국균학회지
2011 .01
-
Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes
Mycobiology
2006 .01
-
Genetic transformation of oriental Cymbidium by a modified Agrobacterium infection method
한국생명과학회 학술발표회
2001 .01
-
Genetic transformation of oriental Cymbidium by a modified Agrobacterium infection method
한국생명과학회 학술발표회
2000 .01
-
Agrobacterium-Mediated Transformation System for Large-Scale Producion of Transgenic Chinese Cabbage (Brassica rapa L. ssp. pekinensis) Plants for Insertional Mutagenesis
식물학회지
2004 .01
-
Construction of Small Binary Vectors for Agrobacterium-Mediated Transformation in Plants
식물학회지
1999 .01
-
Antisense expression of a staygreen gene (SGR) delays leaf senescence in creeping bentgrass
Rapid communication in photoscience : RCP
2014 .01
-
Agrobacterium을 이용한 형질전환에서 sonication과 vir 유전자들의 효과 ( Effect of Sonication and vir Genes on Transient Gene Expression in Agrobacterium-Mediated Transformation )
생명과학회지
2001 .08
-
Molecular Breeding for Plant Disease Resistance : Prospects and Problems
The Plant Pathology Journal
2001 .02
-
A Comparison of the Phenotypic and Genetic Stability of Recombinant Trichoderma spp. Generated by Protoplast- and Agrobacterium-Mediated Transformation
The Journal of Microbiology
2006 .08
-
Current Technologies and Related Issues for Mushroom Transformation
Mycobiology
2015 .01
-
Efficient Transformation of Korean Rice Cultivars (Oryza sativa L.) Mediated by Agrobacterium tumefaciens
식물학회지
1998 .01
-
Two-Dimensional Ordination 分析法에 依한 除草劑殺草 Spectrum 分類와 效果的인 使用法
한국식물보호학회지
1983 .06
-
An Efficient and Stable Method for the Transformation of Heterogeneous Genesinto Cephalosporium acremonium Mediated by Agrobacterium tumefaciens
Journal of Microbiology and Biotechnology
2005 .01
-
Transformation and Inheritance of Bt Genes in Gossypium hirsutum
식물학회지
2008 .01
-
Agrobacterium을 이용한 팽이 버섯 주름조직의 형질전환
한국균학회지
2010 .01
-
Binary Vectors for Efficient Transformation of Rice
식물학회지
1999 .01
-
Agrobacterium 숙주 및 Ti plasmid의 종류에 따른 Agrobacterium의 숙주범위조사
한국동물학회 학술대회
1995 .01
-
Screening of the highly regenerable lines and efficient transformation system using Agrobacterium in lettuce ( Lactuca Sativa L.)
한국생명과학회 심포지움
2005 .04
-
Method development for non-selective herbicides in weeds
한국분석과학회 학술대회
2015 .11
이 논문의 저자 정보
최근 본 자료
댓글(0)
0