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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 171 - 175 (5page)
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초록· 키워드
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Bursaphelenchus xylophilus has been recognized as a causal pathogen of pine wilt disease (PWD). In order to identify relevant molecular biomarkers, we selected a variable region of endo-${\beta}$-1,4-glucanase (${\beta}14-gcn$) which was cloned from the cDNA of B. xylophilus and B. mucronatus. We then identified three novel ${\beta}14-gcn$ isoforms: $Bm-{\beta}14-gcn\;and\;Bm-{\beta}14-gcni$ from B. mucronatus, and $Bx-{\beta}14-gcn$ from B. xylophilus. $Bm-{\beta}14-gcn\;and\;Bx-{\beta}14-gcn$ were found to be identical, whereas $Bm-{\beta}14-gcni$ was unique to B. mucronatus. These isoforms are Bursaphelenchus-specific and may be employed as molecular markers for the diagnosis of PWD. Our phylogenetic analysis showed that these ${\beta}14-gcns$ from Bursaphelenchus species were associated most closely with fungal ${\beta}14-gcns$.
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