지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.9
- 수록면
- 266 - 272 (7page)
- DOI
- 10.7841/ksbbj.2020.35.3.266
이용수
초록· 키워드
오류제보하기
Electrical stimulation (ES) is known to guide the development and regeneration of many tissues. Cartilage has no direct blood supply, thus it has little or no capacity to repair itself. Articular cartilage is to provide a smooth lubricating surface to the joints and facilitates the transfer of loads with a low frictional coefficient. Meniscus is cartilaginous tissues that provide structural integrity to the knee when it undergoes tension and torsion. We tried to isolate and characterize chondrocytes from articular cartilage (Ar-chon) and meniscus (Me-chon) of dogs. Also, we exposed Me-chon to ES and assessed changes in the chondrogenic potential. As results, Ar-chon and Me-chon had fibroblasts-like morphology. The proliferative capacity of Me-chon was higher than those of Ar-chon in the early passage. Both cells expressed cell surface markers such as CD29, CD44, and CD90, but did not express CD34, CD117, and MHC-2. ES was applied to Mechon cultured under condition of high-density micromass under ES of 10 V/㎝, with duration of 10 ㎳ and a frequency of 2 ㎐ for three days. And then, gene expression of chondrogenic markers such as type II collage alpha 1, Aggrecan, and SOX9 was analyzed. As a result, we observed the upregulated expression level of chondrogenic markers of Me-chon by specific ES in absence of exogenous factors. Also, we found that ES significantly upregulated expression level of heat shock protein, which affects cartilage formation. Taken together, ES-driven Me-chon is thought to be useful for basic and therapeutic research applications in the early passage.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSIONS
4. CONCLUSION
REFERENCES
참고문헌 (33)
참고문헌 신청
Hunziker, E. B. / 2002 / Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects / Osteoarthritis Cartilage 10:432~463
Madeira, C. / 2015 / Advanced cell therapies for articular cartilage regeneration / Trends Biotechnol 31:35~42
Negoro, T. / 2018 / Trends in clinical trials for articular cartilage repair by cell therapy / NPJ Regen. Med 3:17
Hardy, J. G. / 2015 / Electrical stimulation of human mesenchymal stem cells on conductive nanofibers enhances their differentiation toward osteogenic outcomes / Macromol. Rapid Commun 36:1884~1890
Hernández-Bule, M. L. / 2014 / Electric stimulation at 448 kHz promotes proliferation of human mesenchymal stem cells / Cell Physiol. Biochem. 34:1741~1755
Madeira, C. / 2015 / Advanced cell therapies for articular cartilage regeneration / Trends Biotechnol 31:35~42
Negoro, T. / 2018 / Trends in clinical trials for articular cartilage repair by cell therapy / NPJ Regen. Med 3:17
Hardy, J. G. / 2015 / Electrical stimulation of human mesenchymal stem cells on conductive nanofibers enhances their differentiation toward osteogenic outcomes / Macromol. Rapid Commun 36:1884~1890
Hernández-Bule, M. L. / 2014 / Electric stimulation at 448 kHz promotes proliferation of human mesenchymal stem cells / Cell Physiol. Biochem. 34:1741~1755
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UCI(KEPA) : I410-ECN-0101-2020-470-001290326