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논문 기본 정보

자료유형
학술저널
저자정보
Suwimon Kanchanasuta (Mahidol University) Verawat Champreda (National Center for Genetic Engineering and Biotechnology) Nipon Pisutpaisal (King Mongkut’s University of Technology North Bangkok) Chatchawal Singhakant (Mahidol University)
저널정보
대한환경공학회 Environmental Engineering Research Environmental Engineering Research 제26권 제4호
발행연도
2021.8
수록면
43 - 50 (8page)

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초록· 키워드

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Bio-succinic acid is a commodity chemical with potent application in bioplastic and food industries which can be produced from renewable resources. In this study, bioprocess for production of bio-succinic from glycerol by Actinobacillus succinogenes was studied. The maximum succinic acid concentration in small-scale serum bottle experiments was 6.8 and 6.5 g/L using pure and crude glycerol, respectively, with supplemented MgCO₃. The ratio of acetic acid to succinic acid (AA/SA) implied the increased carbon flux to the C₄ pathway using crude glycerol supplemented with MgCO₃ compared to that with CaCO₃.The carbonate salts tended to induce C₃ metabolic pathway in fermentation using pure glycerol which was in accordance with the ratio of acetic acid to glycerol (AA/GL). The highest succinic acid concentration of 17.9 g/L from crude glycerol was achieved from batch fermentation in a lab-scale fermenter with the maximum glycerol utilization of 99.9% which were higher than those obtained from fed- batch and semi-continuous processes. Acetic acid tended to increase throughout the fermentation process in fed-batch and semi-continuous operations, which resulted in the lower product yield and substrate utilization efficiency. The time for initial purging of CO₂ showed effects on succinic acid production and internal metabolic pathways. This work provided a basis for process development on bio-succinic acid production from crude glycerol in industry.

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ABSTRACT
1. Introduction
2. Material and Methods
3. Results and Discussion
4. Conclusions
References

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