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논문 기본 정보

자료유형
학술저널
저자정보
Sung-Dug Oh (Rural Development Administration) Seok-Ki Min (Korea Testing & Research Institute) Jae Kwang Kim (Incheon National University) Jung-Ho Park (Korea Research Institute of Bioscience and Biotechnology) Chang-Gi Kim (Korea Research Institute of Bioscience and Biotechnology) Soo Yun Park (Rural Development Administration)
저널정보
충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.47 No.4
발행연도
2020.12
수록면
791 - 802 (12page)

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초록· 키워드

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Theoredoxin (TRX) transgenic soybeans were developed using the human Theoredoxin gene under the control of the β-conglycinin promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. This study was done to assess the acute toxicity of a genetically modified (GM) soybean using the fresh water planktonic crustacean Daphnia magna. The acute toxicity effect of the TRX soybean and non-GM soybean (Gwangan) on D. magna was investigated at different concentrations (0, 156, 313, 625, 1,250, 2,500, and 5,000 mg·L<SUP>-1</SUP>). The TRX soybean used for the test was confirmed to express the TRX/PAT genes by PCR and enzyme-linked immunosorbent assay (ELISA). D. magna feeding tests showed no significant differences in the cumulative immobility or an abnormal response with either the TRX soybean or non-GM soybean. The feeding study showed a similar abnormal response and cumulative immobility of the D. magna between the TRX soybean and Gwangan treatments. Additionally, the 48 h-EC<SUB>50</SUB> values for the TRX and Gwangan soybeans were 755.6 and 778 mg·L<SUP>-1</SUP>, respectively. The soybean NOEC (no observed effect concentration) value for D. magna was suggested to be 156 mg·L<SUP>-1</SUP>. These results suggest that there is no significant difference in toxicity to Daphnia magna between the TRX soybean and its non-GM counterpart.

목차

Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
Reference

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