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논문 기본 정보

자료유형
학술저널
저자정보
Hakjae Chung (Rural Development Administration) Sunyoung Baek (Rural Development Administration) Soojin Sa (Rural Development Administration) Youngshin Kim (Rural Development Administration) Joonki Hong (Rural Development Administration) Eunseok Cho (Rural Development Administration) Jihwan Lee (Rural Development Administration) Seungmin Ha (Rural Development Administration) Jungho Son (Chungnam Techno Park) Seunghwan Lee (Chungnam University) Inchul Choi (Chungnam University) Kyungwoon Kim (Rural Development Administration)
저널정보
충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.47 No.4
발행연도
2020.12
수록면
979 - 985 (7page)

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초록· 키워드

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A wide range of techniques have been developed to separate X or Y- chromosome-bearing sperm. In particular, bovine semen sex-sorted by using flow cytometry based on differences in the amount of DNA between X and Y chromosome bearing sperm is used in dairy farms. The first piglets were produced using sex-sorted sperm 30 years ago. However, sexed sperm have not been commercially available in pigs because the flow cytometry technique is not capable of sorting the high number of sperm required for porcine artificial insemination (AI), and the prolonged exposure to an electrical filed might damage to the DNA in sperm. The purpose of this study was to evaluate a boar sperm sorting method based on magnetic nanoparticles. A flow cytometer assay verified the efficacy of the magnetic nanoparticles (> 90% of sex-sorted sperm). In addition, a duplex polymerase chain reaction (PCR) assay using sex chromosome specific genes including SRY (sex-determining region Y; male), ZFY (zinc finger protein Y-linked; male), and ZFX (zinc finger protein X-linked; female) showed that in vitro fertilized porcine embryos by X and Y-chromosome bearing sperm were 100% female (40/40) and 72% female (35/48), respectively, at 8-cell or morula stages, suggesting that the sex-sorted sperm were fertile. In conclusion, our findings suggest that the sex-sorted method based on magnetic nanoparticles can be utilized for porcine sex-sorted AI.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References

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