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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 126 - 133 (8page)
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초록· 키워드
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Background: 20(S)-protopanaxadiol (20(S)-PPD), one of the aglycone derivatives of major ginsenosides, has been shown to have an anticancer activity toward a variety of cancers. This study was initiated with an attempt to evaluate its anti-cancer activity toward human endometrial cancer by cell and xenograft mouse models.
Methods: Human endometrial cancer (HEC)-1A cells were incubated with different 20(S)-PPD concentrations. 20(S)-PPD cytotoxicity was evaluated using MTT assay. Apoptosis was detected using the annexin V binding assay and cell cycle analysis. Cleaved poly (ADP-ribose) polymerase (PARP) and activated caspase-9 were assessed using western blotting. HEC-1A cell tumor xenografts in athymic mice were generated by inoculating HEC-1A cells into the flank of BALB/c female mice and explored to validate 20(S)-PPD anti-endometrial cancer toxicity.
Results: 20(S)-PPD inhibited HEC-1A cell proliferation in a dose-dependent manner with an IC<SUB>50</SUB> value of 3.5 μM at 24 h. HEC-1A cells morphologically changed after 20(S)-PPD treatment, bearing resemblance to Taxol-treated cells. Annexin V-positive cell percentages were 0%, 10.8%, and 58.1% in HEC-1A cells when treated with 0, 2.5, and 5 μM of 20(S)-PPD, respectively, for 24 h. 20(S)-PPD subcutaneously injected into the HEC-1A cell xenograft-bearing mice three times a week for 17 days manifested tumor growth inhibition by as much as 18% at a dose of 80 mg/kg, which sharply contrasted to controls that showed an approximately 2.4-fold tumor volume increase. These events paralleled caspase-9 activation and PARP cleavage.
Conclusion: 20(S)-PPD inhibits endometrial cancer cell proliferation by inducing cell death via a caspase-mediated apoptosis pathway. Therefore, the 20(S)-PPD-like ginsenosides are endowed with ample structural information that could be utilized to develop other ginsenoside-based anticancer agents.
Methods: Human endometrial cancer (HEC)-1A cells were incubated with different 20(S)-PPD concentrations. 20(S)-PPD cytotoxicity was evaluated using MTT assay. Apoptosis was detected using the annexin V binding assay and cell cycle analysis. Cleaved poly (ADP-ribose) polymerase (PARP) and activated caspase-9 were assessed using western blotting. HEC-1A cell tumor xenografts in athymic mice were generated by inoculating HEC-1A cells into the flank of BALB/c female mice and explored to validate 20(S)-PPD anti-endometrial cancer toxicity.
Results: 20(S)-PPD inhibited HEC-1A cell proliferation in a dose-dependent manner with an IC<SUB>50</SUB> value of 3.5 μM at 24 h. HEC-1A cells morphologically changed after 20(S)-PPD treatment, bearing resemblance to Taxol-treated cells. Annexin V-positive cell percentages were 0%, 10.8%, and 58.1% in HEC-1A cells when treated with 0, 2.5, and 5 μM of 20(S)-PPD, respectively, for 24 h. 20(S)-PPD subcutaneously injected into the HEC-1A cell xenograft-bearing mice three times a week for 17 days manifested tumor growth inhibition by as much as 18% at a dose of 80 mg/kg, which sharply contrasted to controls that showed an approximately 2.4-fold tumor volume increase. These events paralleled caspase-9 activation and PARP cleavage.
Conclusion: 20(S)-PPD inhibits endometrial cancer cell proliferation by inducing cell death via a caspase-mediated apoptosis pathway. Therefore, the 20(S)-PPD-like ginsenosides are endowed with ample structural information that could be utilized to develop other ginsenoside-based anticancer agents.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (27)
참고문헌 신청
Parkin DM / 2005 / Global cancer statistics, 2002 / CA Cancer J Clin 55(2):74~108
Ru W / 2015 / Chemical constituents and bioactivities of Panax ginseng (C. A. Mey.) / Drug Discov Ther 9(1):23~32
이정윤 / 2015 / Controversies in the management of endometrial cancer: a survey of the Korean Gynecologic Oncology Group / Journal of Gynecologic Oncology 26(4):277~283
Jitendra Upadhyaya / 2016 / Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea / Journal of Ginseng Research 40(2):105~112
Wang W / 2007 / Determination of ginsenoside Rd in dog plasma by liquid chromatographymass spectrometry after solid-phase extraction and its application in dog pharmacokinetics studies / J Chromatogr Analyt Technol Biomed Life Sci
Ru W / 2015 / Chemical constituents and bioactivities of Panax ginseng (C. A. Mey.) / Drug Discov Ther 9(1):23~32
이정윤 / 2015 / Controversies in the management of endometrial cancer: a survey of the Korean Gynecologic Oncology Group / Journal of Gynecologic Oncology 26(4):277~283
Jitendra Upadhyaya / 2016 / Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea / Journal of Ginseng Research 40(2):105~112
Wang W / 2007 / Determination of ginsenoside Rd in dog plasma by liquid chromatographymass spectrometry after solid-phase extraction and its application in dog pharmacokinetics studies / J Chromatogr Analyt Technol Biomed Life Sci
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