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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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In this study, we describe the development of a new high-performance liquid chromatography (HPLC) method for the simultaneous analysis of six bioactive compounds (including gallic acid, epicatechin 3-gallate, quercitrin, afzelin, quercetin, and kaempferol) from Orostachys japonicus. The extraction method was investigated and optimization of the extraction time (min), solvent composition (%), and solvent to material ratio were conducted. As a result, 30 min extraction with 50% methanol and 40:1 mL/g of solvent: material ratio achieved the highest extraction efficiency with a yield of 3.32 mg/g. Furthermore, the developed HPLC method was validated and the correlation coefficient (R) values were within the satisfactory range of 0.9995-0.9999 over the linearity range of 1.53-417 μg/mL. The limit of detection and limit of quantification for the six active components were between 0.03-0.08 μg/mL and 0.08-0.26 μg/mL, respectively. With these newly optimized and developed methods, four batches of O. japonicus were analyzed to confirm the high extraction efficiency of the method and the feasibility of an application.
목차
Abstract
1. Introduction
2. Experimental
3. Results and Discussion
4. Conclusions
References
참고문헌 (29)
참고문헌 신청
J. H. Lee, S. J. Lee, S. Park, H. K. Kim, W. Y. Jeong, J. Y. Choi, N.-J. Sung, W. S. Lee, C.-S. Lim and G.-S. Kim, Food Chem., 124(4), 1627-1633 (2011).
S.-J. Lee, J.-K. Seo, J.-H. Shin, H.-J. Lee and N.-J. Sung, J. Korean Soc. Food Sci. Nutr., 37(5), 605-611 (2008).
M.-J. Ko, H.-H. Nam and M.-S. Chung, Sci. Rep., 10(1), 1-10 (2020).
D. S. Im, J.-M. Lee, J. Lee, H. J. Shin, K. T. No, S.-H. Park and K. Kim, Acta Biochim. Biophys. Sin., 49(10), 945-953 (2017).
J. C. Park, W. D. Han, J. R. Park, S. H. Choi and J. W. Choi, J. Ethnopharmacol., 102(3), 313-318 (2005).
S.-J. Lee, J.-K. Seo, J.-H. Shin, H.-J. Lee and N.-J. Sung, J. Korean Soc. Food Sci. Nutr., 37(5), 605-611 (2008).
M.-J. Ko, H.-H. Nam and M.-S. Chung, Sci. Rep., 10(1), 1-10 (2020).
D. S. Im, J.-M. Lee, J. Lee, H. J. Shin, K. T. No, S.-H. Park and K. Kim, Acta Biochim. Biophys. Sin., 49(10), 945-953 (2017).
J. C. Park, W. D. Han, J. R. Park, S. H. Choi and J. W. Choi, J. Ethnopharmacol., 102(3), 313-318 (2005).
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