지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Oleanane-type triterpenoids exist as secondary metabolites in various plants. In particular, soyasaponin, an oleanane-type triterpenoid, is abundant in the hypocotyl of soybean, one of the most widely cultivated crops in the world. Depending on their chemical structure, soyasaponins are categorized as group A saponins or group DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) saponins. The different soyasaponin chemical structures present different health functionalities and taste characteristics. However, conventional phenotype screening of soybean requires a substantial amount of time for functionality of soyasaponins. Therefore, we attempted to use liquid chromatography with a photodiode array detector and tandem mass spectrometry (LC-PDA/MS/MS) for accurately predicting the phenotype and chemical structure of soyasaponins in the hypocotyl of five common soybean natural mutants. In this method, the aglycones (soyasapogenol A [SS-A] and soyasapogenol B [SS-B]) were detected after acid hydrolysis. These results indicated that the base peak and fragmentation differ depending on the chemical structure of soyasaponin with aglycone. Thus, a fragmentation database can help predict the chemical structure of soyasaponins in soyfoods and plants.
목차
Abstract
1. Introduction
2. Experimental
3. Results and Discussion
4. Conclusions
References
참고문헌 (11)
참고문헌 신청
G. R. Fenwick, K. R. Price, C. Tsukamoto and K. Okubo, ‘In Toxic Substances in Crop Plants’, 1st Ed., p285-327, Woodhead Publishing, United Kingdom, 1991.
W. A. Oleszek and Z. Bialy, Journal of Chromatography A, 1112, 78-91 (2006).
P. Krishnamurthy, C. Tsukamoto, Y. Takahashi, Y. Hongo, R. J. Singh, J. D. Lee and G. Chung, Bioscience, Biotechnology, and Biochemistry, 78(12), 1988-1996 (2014).
P. Krishnamurthy, Y. Fujisawa, Y. Takahashi, H. Abe, K. Yamane, K. Mukaiyama, H. R. Son, S. Hiraga, A. Kaga, T. Anai, C. Tsukamoto and M. Ishimoto, Plant Cell Physiol., 60(5), 1082-1097 (2019).
X. Wang and L. Li, Mass Spectrometry Letters, 11(2), 17-24 (2020).
W. A. Oleszek and Z. Bialy, Journal of Chromatography A, 1112, 78-91 (2006).
P. Krishnamurthy, C. Tsukamoto, Y. Takahashi, Y. Hongo, R. J. Singh, J. D. Lee and G. Chung, Bioscience, Biotechnology, and Biochemistry, 78(12), 1988-1996 (2014).
P. Krishnamurthy, Y. Fujisawa, Y. Takahashi, H. Abe, K. Yamane, K. Mukaiyama, H. R. Son, S. Hiraga, A. Kaga, T. Anai, C. Tsukamoto and M. Ishimoto, Plant Cell Physiol., 60(5), 1082-1097 (2019).
X. Wang and L. Li, Mass Spectrometry Letters, 11(2), 17-24 (2020).
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