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논문 기본 정보

자료유형
학술저널
저자정보
Han Yan (Harbin Institute of Technology) Jianzheng Li (Harbin Institute of Technology) Jia Meng (Harbin Institute of Technology) Xin Wang (Harbin Institute of Technology) Lianggang Tang (Harbin Institute of Technology) Avinash Kumar Jha (Harbin Institute of Technology)
저널정보
대한환경공학회 Environmental Engineering Research Environmental Engineering Research 제27권 제4호
발행연도
2022.8
수록면
137 - 144 (8page)

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Nitrite-dependent anaerobic methane oxidation (n-DAMO) has been regarded as a promising effective approach to nitrogen removal from wastewater. However, n-DAMO bacteria are very difficult to be enriched in biological wastewater treatment processes. An anaerobic sequencing batch reactor (AnSBR) was introduced in the present study for the enrichment of n-DAMO bacteria with cornfield soil as inoculum. Fed with nitrite (NO₂<SUP>−</SUP>) and methane as the specific substrates, a NO₂<SUP>−</SUP> load removal as high as 46.16 mg/(L·d) was obtained in the AnSBR since the 232<SUP>nd</SUP> day of enrichment culturing, though the relative abundance of Candidatus Methylomirabilis referring to n-DAMO bacteria was 2.37% in the acclimatized mixed culture. High-throughput sequencing of the obtained mixed culture revealed that the community structure was complex with the coexistence of n-DAMO bacteria, methanotrophs, heterotrophic denitrifying bacteria and hydrolytic fermentation bacteria. Analysis of interactions among the prevalent microbial populations suggested that Candidatus Methylomirabilis had played a key role in the metabolic network of the mixed culture. The research work presented a novel approach to the enrichment of n-DAMO bacteria from cornfield soil and was helpful in understanding the role of n-DAMO bacteria in complex matrices.

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ABSTRACT
1. Introduction
2. Materials and Methods
3. Results and Discussion
4. Conclusions
References

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