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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.7
- 수록면
- 206 - 206 (1page)
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Immortal cell lines are suitable for diverse in vitro experiments, which are prerequisite for effective animal testing. Callithrix jacchus, the common marmoset is an important non-human primate model used for establishing various human diseases. Several strategies generating immortal marmoset cell lines have been reported, but only a limited number of immortalized marmoset cells are now available. Previous studies using gene knockout mice have shown that p53- or Ink4a/Arf-deficient mouse embryonic fibroblasts (MEFs) are immortal and do not express any signs of replicative senescence. In this study, we utilized lentiviruses expressing CRISPR-Cas9 endonucleases and showed that their gene targeting can be convenient procedures for the immortalizatio ... 전체 초록 보기
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