NMR-based Metabolomic Responses of Zebrafish (Danio Rerio) by Fipronil Exposure

이수진; 구남형; 김원식; 김병엽; 정순홍; 김영준

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자료유형: 학술저널

저자정보: 이수진 (부산대학교) 구남형 (한국지질자원연구원) 김원식 (한국지질자원연구원) 김병엽 (한국지질자원연구원) 정순홍 (한국지질자원연구원) 김영준 (한국지질자원연구원)

저널정보: 한국자기공명학회 Journal of the Korean Magnetic Resonance Society Journal of the Korean Magnetic Resonance Society 제24권 제4호

발행연도: 2020.1

수록면: 104 - 116 (13page)

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Fipronil, the phenylpyrazole insecticide, is effective and used in various fields. Especially, fipronil was reliable because it was known to be specific on invertebrate animals than vertebrate animals including mammals. However, fipronil had potential risks that affect vertebrate animals as it blocks the gamma-aminobutyric acid (GABA) receptors that also exists in vertebrates as well as invertebrates. Therefore, it was necessary that harmful effects of fipronil on vertebrates are clarified. For this purpose, the zebrafish (Danio rerio) were used on behalf of vertebrate animals in present study. The zebrafish were exposed to 5 μg/L, 25 μg/L, and 50 μg/L of fipronil during 12, 24 and 72 hours. To closely observe toxic process, 12 hours and 24 hours of additional time point were set in the exposure test. Nuclear magnetic resonance (NMR)-based metabolomics is an approach to detect metabolic changes in organism resulted from external stimuli. In this study, NMR-based metabolomics showed the metabolic changes in zebrafish caused by fipronil exposure. Metabolic analysis revealed that fipronil interfered with energy metabolism and decreased the antioxidant ability in zebrafish. Antioxidant ability decline was remarkable at high exposure concentration. In addition, metabolic analysis results over time suggested that reactions for alleviating the excessive nerve excitation occurred in zebrafish after fipronil exposure. Through this study, it was elucidated that the adverse effects of fipronil on vertebrate animals are evident. The risk of fipronil on vertebrates can be no longer ignored. Moreover, this study has a meaning of practically necessary research for organism by examining the effects of fipronil at low concentrations existed in real environment.

#High resolution-magic angle spinning #nuclear magnetic resonance spectroscopy #metabolomics #Danio rerio #Fipronil

참고문헌 (52)

참고문헌 신청

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