지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Isolation and separation of biological nanoparticles, such as cells and extracellular vesicles, are important techniques for their characterization. Dielectrophoresis (DEP) based on microfluidic chips is an effective method to isolate and separate the nanoparticles. However, the electrodes of the DEP chips are electrolyzed by the electrical signals applied to the nanoparticles. Thus, the isolation/separation efficiency of the nanoparticles is reduced considerably. Through this study, we developed a microfluidic DEP chip for reliable isolation/separation of nanoparticles and developed a passivation technique for the protection of the DEP chip electrodes. The electrode passivation process was designed using a hydrogel and the stability of the hydrogel passivation layer was verified. The fabricated DEP chip and the proposed passivation technique were used for the collection and dispersion of the fluorescent polystyrene nanoparticles. The proposed chip and the technique for isolation and separation of nanoparticles can be leveraged in various bioelectronic applications.
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참고문헌 (12)
참고문헌 신청
T. Salafi / 2017 / Advancements in microfluidics for nano particle separation / Lab Chip 17:11~33
H. Shao / 2018 / New Technologies for Analysis of Extracellular Vesicles / Chem. Rev. 118(4):1917~1950
M. Salih / 2014 / Urinary Extracellular Vesicles and the Kidney : Biomarkers and Beyond / Am. J. Physiol. -Renal Physiol. 306:1251~1259
C. Gardiner / 2016 / Techniques Used for the Isolation and Characterization of Extracellular Vesicles : Results of a Worldwide Survey / J. Extracell. Vesicle. 5:1~6
D. Kang / 2008 / Proteomic Analysis of Exosomes from Human Neural Stem Cells by Flow Field-Flow Fractionation and Nanoflow Liquid Chromatography-Tandem Mass Spectrometry / J. Proteome Res. 7(8):3475~3480
H. Shao / 2018 / New Technologies for Analysis of Extracellular Vesicles / Chem. Rev. 118(4):1917~1950
M. Salih / 2014 / Urinary Extracellular Vesicles and the Kidney : Biomarkers and Beyond / Am. J. Physiol. -Renal Physiol. 306:1251~1259
C. Gardiner / 2016 / Techniques Used for the Isolation and Characterization of Extracellular Vesicles : Results of a Worldwide Survey / J. Extracell. Vesicle. 5:1~6
D. Kang / 2008 / Proteomic Analysis of Exosomes from Human Neural Stem Cells by Flow Field-Flow Fractionation and Nanoflow Liquid Chromatography-Tandem Mass Spectrometry / J. Proteome Res. 7(8):3475~3480
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