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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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The bulb onion (Allium cepa L.), one of the most important vegetables worldwide, contains various functional compounds suchas quercetin, allicin, and flavonoids. Red onions are rich in anthocyanins, a flavonoid that is a functional phytochemical with antioxidativeand anticancer activities. In the previous study, two quantitative trait loci (QTLs) (qAC4.1 and qAC4.2) controlling the anthocyanin contentwere identified on chromosome 4 in an F2 population derived from a cross between A. cepa ‘SP3B’ and ‘H6’. In this study, we developedsingle nucleotide polymorphism-based high-resolution melting (HRM) markers linked to QTLs qAC4.1 and qAC4.2. In addition, we constructeda new genetic linkage map of chromosome 4 using HRM markers and performed a QTL analysis. The QTL qAC4.1 was false, while qAC4.2was a major QTL. The QTL peak position, logarithm of the odds value, and phenotypic variance explained of qAC4.2 was 53.6 cM, 7.45, and 22.51%,respectively. Four HRM markers (AC4.2_65336.1_1123-HRM, AC4.2_53230.3_454-HRM, AC4.2_11999.1_756-HRM, and AC4.2_14596.1_345-HRM)within the QTL region of qAC4.2 were developed in this study. The average anthocyanin content of B (homozygous paternal) genotypes washigher than that of A (homozygous maternal) and H (heterozygous) genotypes for all markers. Consequently, these markers will be useful formarker-assisted selection to develop onion cultivars with high anthocyanin content.
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참고문헌 (41)
참고문헌 신청
Agati G / 2020 / Are flavonoids effective antioxidants in plants? Twenty years of our investigation / Antioxidants 9:1098
Bresadola L / 2020 / Estimating and accounting for genotyping errors in RAD-seq experiments / Mol Ecol Resour 20:856~870
Cho Y / 2021 / Construction of a high-resolution linkage map and chromosomal localization of the loci determining major qualitative traits in onion(Allium cepa L. ) / Euphytica 217:17
Choi Y / 2020 / Construction of an onion (Allium cepa L.) genetic linkage map using genotyping-bysequencing analysis with a reference gene set and identification of QTLs controlling anthocyanin synthesis and content / Plants 9:616
Cramer CS / 1999 / Morphological, biochemical, and molecular markers in onion / HortScience 34:589~593
Bresadola L / 2020 / Estimating and accounting for genotyping errors in RAD-seq experiments / Mol Ecol Resour 20:856~870
Cho Y / 2021 / Construction of a high-resolution linkage map and chromosomal localization of the loci determining major qualitative traits in onion(Allium cepa L. ) / Euphytica 217:17
Choi Y / 2020 / Construction of an onion (Allium cepa L.) genetic linkage map using genotyping-bysequencing analysis with a reference gene set and identification of QTLs controlling anthocyanin synthesis and content / Plants 9:616
Cramer CS / 1999 / Morphological, biochemical, and molecular markers in onion / HortScience 34:589~593
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