Evaluation of different molecular methods for detection of Senecavirus A and the result of the antigen surveillance in Korea during 2018

JinHwa Heo; Min-Jung Lee; HyunJoo Kim; SuKyung Lee; Jida Choi; Hae-Eun Kang; Hyang-Mi Nam; JinJu Nah

추천

검색

자료유형: 학술저널

저자정보: JinHwa Heo (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon) Min-Jung Lee (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon) HyunJoo Kim (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon) SuKyung Lee (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon) Jida Choi (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon) Hae-Eun Kang (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon) Hyang-Mi Nam (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon) JinJu Nah (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon)

저널정보: 한국동물위생학회(구 한국가축위생학회) 한국가축위생학회지 한국가축위생학회지 제44권 제1호

발행연도: 2021.1

수록면: 15 - 19 (5page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Senecavirus A (SVA), previously known as Seneca Valley virus, can cause vesicular disease and neonatal losses in pigs that is clinically indistinguishable from foot-and-mouth disease virus (FMDV). After the first case report in Canada in 2007, it had been restrictively identified in North America including United States. But, since 2015, SVA emerged outside North America in Brazil, and also in several the Asian countries including China, Thailand, and Vietnam. Considering the SVA occurrence in neighboring countries, there has been a high risk that Korea can be introduced at any time. In particular, it is very important in terms of differential diagnosis in the suspected case of vesicular diseases in countries where FMD is occurring. So far, several different molecular detection methods for SVV have been published but not validated as the reference method, yet. In this study, seven different molecular methods for detecting SVA were evaluated. Among them, the method by Flowler et al, (2017) targeted to 3D gene region with the highest sensitivity and no cross reaction with other vesicular disease agents including FMDV, VSV and SVD, was selected and applied further to antigen surveillance of SVA. A total of 245 samples of 157 pigs from 61 farms submitted for animal disease diagnose nationwide during 2018 were tested all negative. In 2018, no sign of SVA occurrence have been confirmed in Korea, but the results of the surveillance for SVA needs to be continued and accumulated at a high risk of SVA in neighboring countries.

#Senecavirus #Senecavirus A #Seneca valley virus #SVA

참고문헌 (17)

참고문헌 신청

Agnol AMD / 2017 / A TaqMan-based qRT-PCR assay for Senecavirus A detection in tissue samples of neonatal piglets / Molecular and Cellular Probes 33:28~31

Arzt J / 2019 / First detection and genome sequence of Senecavirus A in Vietnam / Microbiology Resource Announcements 8(3)

Bracht AJ / 2016 / Real-time reverse transcription PCR assay for detection of Senecavirus A in swine vesicular diagnostic specimens / PLoS One 11(1):e0146211

Feronato C / 2018 / Development and evaluation of a nested-PCR assay for Senecavirus A diagnosis / Tropical Animal Health and Production 50(2):337~344

Fowler VL / 2017 / Development of a novel real-time RT-PCR assay to detect Seneca Valley virus-1associated with emerging cases of vesicular disease in pigs / Journal of Virological Methods 239:34~37

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!