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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 29 - 29 (1page)
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A large number of diverse microbes exist in the environment, and their functions have been gradually revealed through culture-based and sequence-based approaches. Especially, the development of next-generation sequencers has enabled the accumulation of genome big-data and the construction of draft genomes is progressing rapidly. However, despite this progress, the diversity of environmental microbes is still overwhelming. Therefore, there is a need for an analysis method that can selectively and efficiently collect microbes with useful functions from microbial populations. Here, we have developed a new single-cell analysis platform for screening beneficial microbes by a combination of droplet microfluidics and Raman-micro spectroscopy.
Droplet microfluidics is a technique for generating monodispersed water in oil droplets in a high-throughput manner. By applying droplets as reaction vessels, we have developed a high-throughput single-cell genome sequencing method, which could be applied to comparative genome analysis of microbes at the strain level. On the other hand, Raman micro-spectroscopy enables label-free detection of substances and real-time imaging of cellular components at single-cell level. Furthermore, we have applied ... 전체 초록 보기
Droplet microfluidics is a technique for generating monodispersed water in oil droplets in a high-throughput manner. By applying droplets as reaction vessels, we have developed a high-throughput single-cell genome sequencing method, which could be applied to comparative genome analysis of microbes at the strain level. On the other hand, Raman micro-spectroscopy enables label-free detection of substances and real-time imaging of cellular components at single-cell level. Furthermore, we have applied ... 전체 초록 보기
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