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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 543 - 543 (1page)
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The optimization of culture conditions is a necessary step in biosynthesis after an expression system has been designed. This ensures that engineered microbes utilize media efficiently and ensure the healthy balance between cell growth and high expression of recombinant protein. Green fluorescence protein (GFP) was used as a reporter gene and is not toxic to the cell, however, viral fusion peptide is known to be toxic to cell growth. In this study, we determine the culture conditions (temperature and shaking speed) which will result in optimal GFP and FP expression with high protein yield without compromising cell growth. Of the cultures studied, three batches harbored the single-plasmid co-ex ... 전체 초록 보기
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