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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 307 - 315 (9page)
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초록· 키워드
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Knowledge of genetic resources is essential for breeders to create new crop varieties with improved characteristics. Inthis respect, mutant populations may conveniently provide a powerful tool for identifying new functional genes. Therefore, we used theMicro-Tom tomato variety, which has a reduced size and a relatively short life-cycle compared to other commercial tomato cultivars,to construct a mutant population using gamma-ray radiation as a mutagen. To determine the optimal mutagenic intensity of gamma raysfor tomato, dry seeds of Micro-Tom were irradiated with gamma-rayintensities from 0 to 1000 gray (Gy) with increments of 100 Gy. The germination rate of mutagenized seeds (M1 seeds) on MS media was not affected by the tested gamma-ray intensity range. However, seedling growth was severely reduced with increasing irradiation. Seedling growth rate at eight days after germinationshowed that the median gamma-ray doses for hypocotyl and root elongation were 600 and 300-400 Gy, respectively. The survival testfor 300, 400, and 500 Gy-treated M1seeds showed that survival rates significantly decreased with increasing irradiation. The survivalrate of 400 Gy-radiated seeds was 48%, while that of 500 Gy-radiated seeds was only 25%, compared with the control treatment. Therefore, we concluded that gamma-ray irradiation at 300-400 Gy is best for tomato mutagenesis. To find new mutants, M2 seedsproduced by M1plants were grown. We found several mutants, including plants with varying cotyledon number, variegated or redleaves, and green hypocotyls.
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참고문헌 (20)
참고문헌 신청
Mayta ML / 2019 / Expression of a chloroplasttargeted cyanobacterial flavodoxin in tomato plants increases harvest index by altering plant size and productivity / Front. Plant Sci 10:1432
Pailles Y / 2020 / Diverse traits contribute to salinity tolerance of wild tomato seedlings from the Galapagos islands / Plant Physiol 182:534~546
Pereira JA / 2018 / The Arabidopsis elongator subunit ELP3 and ELP4 confer resistance to bacterial speck in tomato / Front. Plant Sci 9:1066
Perez-Fons L / 2014 / A genome-wide metabolomic resource for tomato fruit from Solanum pennellii / Sci. Rep 4:1~8
Pulungan SI / 2018 / SlLAX1 is required for normal leaf development mediated by balanced adaxial and abaxial pavement cell growth in tomato / Plant Cell Physiol 59:1170~1186
Pailles Y / 2020 / Diverse traits contribute to salinity tolerance of wild tomato seedlings from the Galapagos islands / Plant Physiol 182:534~546
Pereira JA / 2018 / The Arabidopsis elongator subunit ELP3 and ELP4 confer resistance to bacterial speck in tomato / Front. Plant Sci 9:1066
Perez-Fons L / 2014 / A genome-wide metabolomic resource for tomato fruit from Solanum pennellii / Sci. Rep 4:1~8
Pulungan SI / 2018 / SlLAX1 is required for normal leaf development mediated by balanced adaxial and abaxial pavement cell growth in tomato / Plant Cell Physiol 59:1170~1186
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