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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 35 - 42 (8page)
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초록· 키워드
오류제보하기
The Vibrio species causing major diseases in Litopenaeus vannamei are Vibrio harveyi, Vibrio alginolyticus, and Vibrio parahaemolyticus. For multiplex PCR primers, YeaD was used to detect the three Vibrio species. Bioinformatic analysis such as MultiPLX and primer-BLAST was used to design stable and species-specific multiplex PCR primers. Multiplex PCR results showed clear band patterns with bands at 185 bp for V. alginolyticus, 396 bp for V. harveyi, 805 bp for V. arahaemolyticus, and 596 bp for common Vibrio species. The minimum concentration of DNA was measured by PCR; the value for V. alginolyticus was 0.1 ng, that of V. harveyi was 0.03 ng, and that of V. parahaemolyticus was 0.003 ng. Taken together, YeaD showed stability and specificity in identifying Vibrio species. Our multiplex PCR amplification method is an effective and inexpensive tool for identifying Vibrio species.
목차
등록된 정보가 없습니다.
참고문헌 (51)
참고문헌 신청
Aftabuddin S / 2017 / First incidence of loose-shell syndrome disease in the giant tiger shrimp Penaeus monodon from the brackish water ponds in Bangladesh / J Appl Anim Res 46:1~8
Aguirre-Guzmán G / 2001 / Differences in the susceptibility of American white shrimp larval substages(Litopenaeus vannamei)to four Vibrio species / J Invertebr Pathol 78:215~219
Ananda RR / 2017 / Pathogenicity profile of Vibrio parahaemolyticus in farmed Pacific white shrimp, Penaeus vannamei / Fish Shellfish Immunol 67:368~381
Annam RA / 2015 / Analysis of engine test and emission test of seaweed biodiesel for sustainable energy / J Chem Pharm Res 7(2):755~760
Biju VN / 2016 / Prevalence of Vibrio infection in Penaeus(Litopenaeus)vannamei farms / Int J Sci Invent Today 5:485~493
Aguirre-Guzmán G / 2001 / Differences in the susceptibility of American white shrimp larval substages(Litopenaeus vannamei)to four Vibrio species / J Invertebr Pathol 78:215~219
Ananda RR / 2017 / Pathogenicity profile of Vibrio parahaemolyticus in farmed Pacific white shrimp, Penaeus vannamei / Fish Shellfish Immunol 67:368~381
Annam RA / 2015 / Analysis of engine test and emission test of seaweed biodiesel for sustainable energy / J Chem Pharm Res 7(2):755~760
Biju VN / 2016 / Prevalence of Vibrio infection in Penaeus(Litopenaeus)vannamei farms / Int J Sci Invent Today 5:485~493
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