지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Background: DEL is an RhD variant that cannot be detected by routine serologic tests because of the extremely low expression of the RhD antigen. Detecting the common genotypes of RHD 1227G＞A and 1222T＞C in Korean DEL is important for safe and efficient blood transfusions. Therefore, in this study, a PCR-restriction enzyme fragment polymorphism (RFLP) method was applied to detect RHD 1227G＞A and 1222T＞C. Methods: DNA extracted from the blood of each segment of 56 units of RhD-negative red blood cell were used. The promoter, exon 7 and exon 9 of RHD, and exon 9 of RHCE were amplified. The PCR products of RHD exon 7, RHD exon 9, and RHCE exon 9 were treated with the restriction enzymes HpyAV and MspI, and the RFLP patterns were observed by electrophoresis. The results of PCR-RFLP of RHD exon 9 were confirmed by PCR-direct sequencing. Results: RHCE exon 9 was amplified in all 56 DNAs. RHD promoters, exon 7, and exon 9 were all amplified in 10 samples, RHD promoter, exon 7, and exon 9 were not amplified in 38 samples, and RHD promoter only was amplified in eight samples. As a result of the RHD exon 9 PCR-RFLP performed on 10 samples with all targets amplified, 10 samples were determined to be 9 samples with 1227G＞A and 1 sample with 1222T＞C. The PCR-RFLP result and the sequencing result were 100% identical. Conclusion: PCR-RFLP using HpyAV and MspI is a reliable and applicable method for detecting RHD 1227G>A and 1222T＞C in serologically RhD negative samples. (Korean J Blood Transfus 2021;32:28-34)
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
한국인에서 RHD 유전자 검사의 임상적 적응증
대한수혈학회지
2024 .04
-
한 가족에서 발견된 Weak D Type 102: 국내 첫 보고
대한수혈학회지
2020 .01
-
D 음성 헌혈자 대상 RHD 유전자 검사 시행 경험
대한수혈학회지
2021 .08
-
RhD 음성/변이형 한국인 환자의 수혈전략
Laboratory Medicine Online
2017 .07
-
약한 D 표현형을 가진 한 환자에서 발견된 weak D type 33: 국내 첫 보고
대한수혈학회지
2019 .01
-
RhD 불일치 조혈모세포이식에서의 수혈 선택
대한수혈학회지
2020 .01
-
틀이동변이 RHD 711delC에 의한 D-변이형 증례 1건
대한수혈학회지
2019 .01
-
RHD 유전자검사로 확진된 Partial D type VI-3 증례
Laboratory Medicine Online
2020 .01
-
Application of Multiplex Ligation-Dependent Probe Amplification Assay for Genotyping Major Blood Group Systems Including DEL Variants in the D-Negative Korean Population
Annals of Laboratory Medicine
2018 .01
-
혈장 수혈에 있어서 RhD 면역예방
대한수혈학회지
2018 .01
-
Noninvasive fetal RHD genotyping using cell-free fetal DNA incorporating fetal RASSF1A marker in RhD-negative pregnant women in Korea
Journal of genetic medicine
2015 .01
-
Primary Anti-D Alloimmunization Induced by “Asian Type” RHD (c.1227G>A) DEL Red Cell Transfusion
Annals of Laboratory Medicine
2015 .01
-
Planned Transfusion of D-Positive Blood Components in an Asia Type DEL Patient: Proposed Modification of the Korean National Guidelines for Blood Transfusion
Annals of Laboratory Medicine
2019 .01
-
ABO-RhD 혈액형 검사와 비예기항체 선별검사를 위한 혈액은행 자동화장비 IH-500과 VISION Max의 평가
Laboratory Medicine Online
2017 .01
-
Molecular and Clinicopathological Features of Gastrointestinal Stromal Tumors in Vietnamese Patients
Journal of Pathology and Translational Medicine
2019 .01
-
면역혈액학 자동화 장비 DAYmate S를 이용한 ABO/RhD 혈액형 검사 및 비예기항체 선별검사의 최소검체량 평가
대한수혈학회지
2018 .01
-
범용 아가로스 겔과 리튬붕산염버퍼를 이용한 PCR-제한효소절편길이다형성법으로 실시한 Kidd (SLC14A1 rs1058396) 유전형 검사
대한수혈학회지
2022 .12
-
Reverse Transcription-PCR-based Sanger Sequencing-confirmed Exon-skipping Effect of a Novel GEN1 Intronic Variant (c.1408+4A>G)
Annals of Laboratory Medicine
2024 .03
-
Use of a High-Throughput Genotyping Platform (OncoMap) for RAS Mutational Analysis to Predict Cetuximab Efficacy in Patients with Metastatic Colorectal Cancer
Cancer Research and Treatment
2017 .01
-
Seamless 클로닝 기법을 이용한 ABO 엑손 2부터 엑손 7을 포함하는 증폭산물 클로닝
대한수혈학회지
2020 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0