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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 277 - 287 (11page)
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초록· 키워드
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This study presents an easy paper-plastic hybrid microfluidic chip fabrication method that explored the use of oven baking a wax-printed pattern for microfluidic channel assembly combined with the use of a double-sided adhesive tape for layer binding. More specifically, the method includes printing of a wax pattern onto the CA film & oven baking of the pattern along with the backed NC membrane at 120°C for 2.5 minutes. The porous nature of the NC membrane allows the wax pattern to enter into its pores during the baking process and binds the backed NC membrane with the CA film. Attachment of the engraved PMMA layer was performed to increase the structural support. The engraved hole of the layer allows the conjugate pad with space for its integration into the system. Moreover, the antibodies required for the integration of the chip with the LFIA were selected by enzyme-linked immunosorbent assay (ELISA). A CA film, an NC membrane, and a PMMA sheet were used to develop a microfluidic chip that can be integrated with a sandwich format lateral flow immunoassay used to detect dengue non-structural 1 protein. The fabricated chip was able to successfully detect a concentration of dengue non-structural 1 protein of at least 84.66 ng/mL.
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참고문헌 (18)
참고문헌 신청
Pollett, S. / 2018 / Understanding dengue virus evolution to support epidemic surveillance and counter-measure development / Infect., Genet. Evol 62:279~295
Viswanath, B. / 2018 / Recent trends in the development of complementary metal oxide semiconductor image sensors to detect foodborne bacterial pathogens / TrAC, Trends Anal. Chem 98:47~57
Xu, Q. Y. / 2016 / Development of a novel protein chip for the detection of bluetongue virus in China / J. Virol. Methods 234:28~33
Kim, J. -H. / 2018 / A novel paper-plastic hybrid device for the simultaneous loop-mediated isothermal amplification and detection of DNA / Mater. Lett 214:243~246
Liu, C. -C. / 2018 / Microfluidic paper-based chip platform for benzoic acid detection in food / Food Chem 249:162~167
Viswanath, B. / 2018 / Recent trends in the development of complementary metal oxide semiconductor image sensors to detect foodborne bacterial pathogens / TrAC, Trends Anal. Chem 98:47~57
Xu, Q. Y. / 2016 / Development of a novel protein chip for the detection of bluetongue virus in China / J. Virol. Methods 234:28~33
Kim, J. -H. / 2018 / A novel paper-plastic hybrid device for the simultaneous loop-mediated isothermal amplification and detection of DNA / Mater. Lett 214:243~246
Liu, C. -C. / 2018 / Microfluidic paper-based chip platform for benzoic acid detection in food / Food Chem 249:162~167
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