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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 306 - 322 (17page)
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Previous culture techniques are often ineffective for providing appropriate conditions to cells grown in vitro for efficient growth and maturation. However, the advent of microfluidic chips allows us to manipulate various factors from co-culturing cells to inducing shear stress and biochemical gradient. The above have all been effectively applied to stem cell engineering, allowing dynamic interactions with other cells and, as a result, acquisition of a more mature state. The introduction of both synthetic and natural hydrogels into the chip provides more precise in vivo-like biophysical and biochemical cues to cells, enabling better recapitulation of the in vivo-like physiological behaviors and further maturation of stem cells even to the scale of organoids. This review addresses fundamental roles of microfluidic chips and hydrogels and how hydrogel-integrated chip systems provide breakthroughs in advanced stem cell engineering.
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참고문헌 (109)
참고문헌 신청
Luo, Y. / 2018 / Three-dimensional hydrogel culture conditions promote the differentiation of human induced pluripotent stem cells into hepatocytes / Cytotherapy 20:95~107
Takayama, K. / 2013 / 3D spheroid culture of hESC/hiPSC-derived hepatocyte-like cells for drug toxicity testing / Biomaterials 34:1781~1789
Lewis, K. J. / 2018 / Epithelialmesenchymal crosstalk influences cellular behavior in a 3D alveolus-fibroblast model system / Biomaterials 155:124~134
Estabridis, H. M. / 2018 / Cell migration in 1D and 2D nanofiber microenvironments / Ann. Biomed. Eng 46:392~403
Korkaya, H. / 2011 / Breast cancer stem cells, cytokine networks, and the tumor microenvironment / J. Clin. Invest 121:3804~3809
Takayama, K. / 2013 / 3D spheroid culture of hESC/hiPSC-derived hepatocyte-like cells for drug toxicity testing / Biomaterials 34:1781~1789
Lewis, K. J. / 2018 / Epithelialmesenchymal crosstalk influences cellular behavior in a 3D alveolus-fibroblast model system / Biomaterials 155:124~134
Estabridis, H. M. / 2018 / Cell migration in 1D and 2D nanofiber microenvironments / Ann. Biomed. Eng 46:392~403
Korkaya, H. / 2011 / Breast cancer stem cells, cytokine networks, and the tumor microenvironment / J. Clin. Invest 121:3804~3809
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