지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 158 - 168 (11page)
이용수
초록· 키워드
오류제보하기
The use of colorimetric bioassays for protein detection is one of the most promising diagnostic approaches, but their relatively poor detection limits have been a critical issue. In this study, we developed an efficient colorimetric bioassay based on antibody-coated peroxidase-mimicking Au nanoparticles (Au NPs) for the detection of prostate-specific antigen (PSA), which is one of the most widely used protein biomarkers for the diagnosis of prostate and breast cancers. Anti-PSA antibody adsorption on the Au NP surface result in 65% of the peroxidasemimicking properties of Au NPs was suppressed to maximize sensitivity of the assay. Anti-PSA antibody adsorption on the Au NP was optimized at 150 µg/mL anti-PSA4 antibody for 1 h incubation, and their surface was blocked with 2% BSA. The experimental conditions of the immunoassay detection were also investigated. A highest assay value was obtained at 10 µg/mL anti-PSA1 capture antibody using 3% BSA for surface blocking and at 2.5 pM anti- PSA4-Ab coated Au NPs for 1 h incubation with PSA antigen. As a result, PSA was detected at a wide range of 0.25 to 2,500 ng/mL with a detection limit of 0.23 ng/mL. The anti-PSA-Ab coated Au NP- based assay was simple, easy to operate, and sensitive. The proposed peroxidase-mimicking anti-PSA Ab-coated Au NPbased assay could be further developed to detect other protein biomarkers.
목차
등록된 정보가 없습니다.
참고문헌 (64)
참고문헌 신청
Masson, J.-F. / Surface Plasmon Resonance Clinical Biosensors for Medical Diagnostics / ACS Sens
Madhavan, B / Combined evaluation of a panel of protein and miRNA serumexosome biomarkers for pancreatic cancer diagnosis increases sensitivity and specificity / Int. J. Cancer
Xu, J. / A gold nanoparticle-based four-color proximity immunoassay for one-step, multiplexed detection of protein biomarkers using ribonuclease H signal amplification / Chem. Commun.
Wang, Y. / Magnetic nanoparticle-enhanced biosensor based on gratingcoupled surface plasmon resonance / Anal. Chem.
Nimse, S.B. / Biomarker detection technologies and future directions / Analyst
Madhavan, B / Combined evaluation of a panel of protein and miRNA serumexosome biomarkers for pancreatic cancer diagnosis increases sensitivity and specificity / Int. J. Cancer
Xu, J. / A gold nanoparticle-based four-color proximity immunoassay for one-step, multiplexed detection of protein biomarkers using ribonuclease H signal amplification / Chem. Commun.
Wang, Y. / Magnetic nanoparticle-enhanced biosensor based on gratingcoupled surface plasmon resonance / Anal. Chem.
Nimse, S.B. / Biomarker detection technologies and future directions / Analyst
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
수소분리 및 정제를 위한 PSA(Pressure Swing Adsorption)시스템 안전성향상에 관한 연구
한국가스학회지
2022 .02
-
국내 PSA 적용을 위한 해외 초기사건 분석방법론 고찰
한국에너지학회 학술발표회
2022 .10
-
국내 PSA 적용을 위한 해외 기기 신뢰도데이터 분석방법론 고찰
한국에너지학회 학술발표회
2022 .10
-
Density Functional Theory Studies of Oxygen Affinity of Small Au Nanoparticles
한국재료학회지
2017 .01
-
PSA와 SAMG 연계성 고찰
한국에너지학회 학술발표회
2016 .04
-
PSA 적용을 위한 최신 신뢰도데이터 평가방법론 및 검증절차 개발 전략
한국에너지학회 학술발표회
2022 .10
-
신규 PSA 안전목표에 대한 국내외 현황 및 개선방향 제언
한국에너지학회 학술발표회
2023 .10
-
가동 원전의 Level 2 PSA 모델 표준화 방안
한국에너지학회 학술발표회
2016 .10
-
ARM PSA 기반 IoT 서비스 개발 모델
한국정보과학회 학술발표논문집
2019 .06
-
고장예지 방법을 이용한 Ageing-dependent PSA 모델 연구
정보 및 제어 논문집
2016 .04
-
고장예지 방법을 이용한 Ageing-dependent PSA 모델 연구
대한전자공학회 학술대회
2016 .04
-
Application of Dynamic Probabilistic Safety Assessment Approach for Accident Sequence Precursor Analysis: Case Study for Steam Generator Tube Rupture
Nuclear Engineering and Technology
2017 .01
-
PSA Off Gas를 사용한 수소 생산 공정 최적화
한국가스학회 학술대회논문집
2024 .05
-
Synthesis and Characterization of Acrylic Pressure Sensitive Adhesives for Li-ion Battery Pack in Electric Vehicle
한국고분자학회 학술대회 연구논문 초록집
2021 .04
-
AIMS-MUPSA software package for multi-unit PSA
Nuclear Engineering and Technology
2018 .01
-
PSA: A Photon Search Algorithm
JIPS(Journal of Information Processing Systems)
2020 .01
-
PSA/RIMS 영향검토와 안전운전에 대한 고찰
한국에너지학회 학술발표회
2023 .10
-
최신 기술을 적용한 Level 2&3 PSA 평가 기술개발 전략
한국에너지학회 학술발표회
2016 .10
-
Chemiluminescing PCA3 and PSA Genes for Prostate Cancer Diagnostics in Urine
한국생물공학회 학술대회
2017 .10
-
격납건물극한내압능력 변화에 따른 Level 2 PSA 영향
한국에너지학회 학술발표회
2016 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0