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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 595 - 604 (10page)
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초록· 키워드
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BACKGROUND: Reconstruction of large eyelid defects remains challenging due to the lack of suitable eyelid tarsus tissue substitutes. We aimed to evaluate a novel bioengineered chitosan scaffold for use as an eyelid tarsus substitute. METHODS: Three-dimensional macroporous chitosan hydrogel scaffold were produced via cryogelation with specific biomechanical properties designed to directly match characteristics of native eyelid tarsus tissue. Scaffolds were characterized by confocal microscopy and tensile mechanical testing. To optimise biocompatibility, human eyelid skin fibroblasts were cultured from biopsy-sized samples of fresh eyelid skin. Immunological and gene expression analysis including specific fibroblast-specific markers were used to determine the rate of fibroblast de-differentiation in vitro and characterize cells cultured. Eyelid skin fibroblasts were then cultured over the chitosan scaffolds and the resultant adhesion and growth of cells were characterized using immunocytochemical staining. RESULTS: The chitosan scaffolds were shown to support the attachment and proliferation of NIH 3T3 mouse fibroblasts and human orbital skin fibroblasts in vitro. Our novel bioengineered chitosan scaffold has demonstrated biomechanical compatibility and has the ability to support human eyelid skin fibroblast growth and proliferation. CONCLUSIONS: This bioengineered tissue has the potential to be used as a tarsus substitute during eyelid reconstruction, offering the opportunity to pre-seed the patient’s own cells and represents a truly personalised approach to tissue engineering.
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등록된 정보가 없습니다.
참고문헌 (28)
참고문헌 신청
Fransen M / 2012 / Non-melanoma skin cancer in Australia / Med J Aust 197:565~568
DiFrancesco LM / 2004 / Upper eyelid reconstruction / Plast Reconstr Surg 114:98e~107e
Milz S / 2005 / An immunohistochemical study of the extracellular matrix of the tarsal plate in the upper eyelid in human beings / J Anat 206:37~45
Ito O / 2001 / Eyelid reconstruction using a hard palate mucoperiosteal graft combined with a V-Y subcutaneously pedicled flap / Br J Plast Surg 54:106~111
Kamiya H / 2003 / Successful use of preserved sclera of eyelid reconstruction / Eur J Dermatol 13:267~271
DiFrancesco LM / 2004 / Upper eyelid reconstruction / Plast Reconstr Surg 114:98e~107e
Milz S / 2005 / An immunohistochemical study of the extracellular matrix of the tarsal plate in the upper eyelid in human beings / J Anat 206:37~45
Ito O / 2001 / Eyelid reconstruction using a hard palate mucoperiosteal graft combined with a V-Y subcutaneously pedicled flap / Br J Plast Surg 54:106~111
Kamiya H / 2003 / Successful use of preserved sclera of eyelid reconstruction / Eur J Dermatol 13:267~271
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