메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류
인문학
인문학 일반 역사학 철학 종교학 어문학 기타 인문학
사회과학
사회과학 일반 경영학 관광학 교육학 군사학 문헌정보학 법학 사회학 신문방송학 심리과학 정치외교학 지리학 행정학 경제학 무역학 인류학 기타 사회과학
자연과학
자연과학 일반 물리학 생물학 생활과학 수학·통계학 천문학 지구과학 화학 기타 자연과학
공학
공학 일반 건축공학 기계공학 산업공학 재료·에너지공학 전기전자공학 조선해양공학 화학공학 컴퓨터학 기타공학
의약학
의학 일반 간호학 수의학 약학 한의학 기초의학 임상의학 기타 의약학
농수해양학
농수해양학 일반 농학 수산학 식품과학 임학 축산학 해상운송학 기타 농수해양학
예술체육학
예술 일반 디자인 미술 미용 사진 음악학 의상 체육 공연매체예술 기타 예술체육학
복합학
학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

ENG

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
Hirak K. Barman (ICAR - Central Institute of Freshwater Aquaculture) Vemulawada Chakrapani (ICAR - Central Institute of Freshwater Aquaculture) Swagat K. Patra (ICAR - Central Institute of Freshwater Aquaculture) Shibani D. Mohapatra (ICAR - Central Institute of Freshwater Aquaculture) Kiran D. Rasal (ICAR - Central Institute of Freshwater Aquaculture) Uday Deshpande (Bioserve India) Swapnarani Nayak (ICAR - Central Institute of Freshwater Aquaculture) Jitendra K. Sundaray (ICAR - Central Institute of Freshwater Aquaculture) Pallipuram Jayasankar (ICAR - Central Institute of Freshwater Aquaculture)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.38 No.11
발행연도
2016.1
수록면
1,061 - 1,076 (16page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
The high-throughput sequencing technology provides a platform for revealing the expressed genes within a tissue at a specific time. The giant freshwater prawn, Macrobrachium rosenbergii, is an economically important species, which is surviving in a wide-range of salinity. In this study, to understand the physiological mechanism of adaptability with respect to moulting and salinity; transcriptome sequencing of larvae and post-larvae of M. rosenbergii was performed using the Illumina GAIIx platform. The generated raw read-data comprised 71,391,946 and 75,276,622 paired-end reads (PE) for larvae and post-larvae respectively. Using CLC bio Genomic Workbench version 7.5 (CGWB), 71.39 million and 75.27 million of each 72 base paired-end, high quality reads were assembled into 43,383 (N50 1852) and 44,960 (N50 1874) transcripts, respectively, for larvae and post-larvae. The nucleotide level annotation of both transcriptomes showed significant similarity with unigenes of closely related species. The Gene Ontology analysis suggested enrichment of transcripts involving several biological processes linked to transcriptional regulation, signal transduction, immune response, ion-binding. Differential gene expression analysis using CGWB and DESeq identified 9680 deregulated genes of which 3454 unigenes were up-regulated and 3068 down-regulated by C1.5 fold (p\0.05) in larval stage compared to post-larval stage. However, in larval stage 938 genes were down regulated and 1599 genes up-regulated by C3 fold with p\0.05. GO enrichment of differentially expressed genes was shown several molecular functions for maintaining homeostasis against salinity stress. To validate the expression patterns, few transcripts were chosen for quantitative real-time PCR that showed the consistency and exactness of our analysis. In addition, we also speculated the enzymatic pathway using KEGG, which depicted that up-regulated genes are involved in several significant metabolic pathways and those are critical for maintaining osmoregulation and linked with metamorphosis. Therefore, we have generated valuable information of salinity tolerant genes in the larval and post larval stage of M. rosenbergii during salt- and freshwater compliances, which will be further harnessed for gene targeting. The present finding would provide the basis for further screening of salt tolerant genes associated markers for selective breeding.

목차

등록된 정보가 없습니다.

참고문헌 (58)

참고문헌 신청
Annadurai RS / 2012 / Next generation sequencing and de novo transcriptome analysis of Costus pictus D. Don, a nonmodel plant with potent anti-diabetic properties / BMC Genomics 13:663 google schola Barman HK / 2012 / Identification and characterization of differentially expressed transcripts in the gills of freshwater prawn (Macrobrachium rosenbergii) under salt stress / ScientificWorldJournal 2012:149361 google schola Chand BK / 2015 / Effect of salinity on survival and growth of giant freshwater prawn Macrobrachium rosenbergii (de Man) / Aquac Rep 2:26~33 google schola Chen K / 2015 / Transcriptome and molecular pathway analysis of the hepatopancreas in the Pacific White Shrimp Litopenaeus vannamei under chronic low-salinity stress / PLoS One 10:e0131503 google schola Delattre M / 2009 / The evolutionary context of robust and redundant cell biological mechanisms / BioEssays 31:537~545 google schola

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0