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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 387 - 395 (9page)
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To identify novel allelic variations in key genes of wheat quality, the present study used the targeting induced local lesions in genomes platform to detect point mutations in target genes. The wheat variety Longfumai 17 was treated by the mutagen ethyl methanesulfonate to produce a bulk M2 generation, and the population included 1122 plants. A total length of 3906.80 kb nucleotides was analyzed, and the average mutation density was 1/244.17 kb. The identified mutations included G>A substitutions (43.75%), C>T substitutions (31.25%), A insertions (12.50%), T insertions (6.25%), and deletions (6.25%). These point mutations led to changes in amino acids and thus the encoded protein sequences, ultimately producing 18.75% of missense mutations, 12.50% of frame shift mutations, 6.25% of nonsense mutations, 25.00% of silent mutations and 37.50% of non-coding region mutations. In the kernel hardness gene Pinb and 3 starch synthesis genes waxy, Agp2 and SSIIa-A, we detected 16 different point mutations in 25 mutant lines. The Pinb gene harbored two missense mutations and a nonsense mutation; the C>T missense mutation resulted in a novel allele, this novel allele and the nonsense mutation alerted protein 3D structure; the waxy gene presented missense and frame shift mutations; the Agp2 gene carried a missense mutation; the SSIIa-A incurred a missense mutation and a frame shift mutation that resulted in premature protein termination. All the frame shift mutations, nonsense mutations and the Pinb novel allele resulted in allelic variation of their corresponding genes, which in turn affected their gene functions. The identified mutant lines can be used as intermediate materials in wheat quality improvement schemes.
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참고문헌 (59)
참고문헌 신청
Ainsworth C / 1995 / Adenosine diphosphate glucose pyrophosphorylase genes in wheat differential expression and gene mapping / Planta 197:1~10
Ashkani S / 2015 / Allele mining strategies-principles and utilisation for blast resistance genes in rice (Oryza sativa L.) / Curr Issues Mol Biol 17:57~74
Beecher B / 2002 / Expression of wild-type pinb sequence in transgenic wheat complements a hard phenotype / Theor Appl Genet 105:870~877
Bovina R / 2014 / Development of a TILLING resource in durum wheat for reverse- and forwardgenetic analyses / Crop Pasture Sci 65:112~124
Campbell J / 2012 / A mutagenesis-derived broad-spectrum disease resistance locus in wheat / Theor Appl Genet 125:391~404
Ashkani S / 2015 / Allele mining strategies-principles and utilisation for blast resistance genes in rice (Oryza sativa L.) / Curr Issues Mol Biol 17:57~74
Beecher B / 2002 / Expression of wild-type pinb sequence in transgenic wheat complements a hard phenotype / Theor Appl Genet 105:870~877
Bovina R / 2014 / Development of a TILLING resource in durum wheat for reverse- and forwardgenetic analyses / Crop Pasture Sci 65:112~124
Campbell J / 2012 / A mutagenesis-derived broad-spectrum disease resistance locus in wheat / Theor Appl Genet 125:391~404
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