Differential MicroRNA Expression Between Gastric Cancer Tissue and Non-cancerous Gastric Mucosa According to Helicobacter pylori Status

이정원; 김나영; 박지현; 김희지; 장현; 김정민; 김진욱; 이동호

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자료유형: 학술저널

저자정보: 이정원 (서울대학교병원) 김나영 (서울대학교) 박지현 (서울대학교) 김희지 (분당서울대학교병원) 장현 (서울대학교병원) 김정민 ((주) 엔에이알센터 (NAR Center)) 김진욱 (서울대학교) 이동호 (서울대학교)

저널정보: 대한암예방학회 대한암예방학회지 대한암예방학회지 제22권 제1호

발행연도: 2017.1

수록면: 33 - 39 (7page)

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Background: MicroRNAs (miRNAs) are key post-translational mechanisms which can regulate gene expression in gastric carcinogenesis. To identify miRNAs responsible for gastric carcinogenesis, we compared expression levels of miRNAs between gastric cancer tissue and non-cancerous gastric mucosa according to Helicobacter pylori status. Methods: Total RNA was extracted from the cancerous regions of formalin-fixed, paraffin-embedded tissues of H. pylori-positive (n =8) or H. pylori-negative (n = 8) patients with an intestinal type of gastric cancer. RNA expression was analyzed using a 3,523 miRNA profiling microarray based on the Sanger miRBase. Validation analysis was performed using TaqMan miRNA assays for biopsy samples from 107 patients consisted of control and gastric cancer with or without H. pylori. And then, expression levels of miRNAs were compared according to subgroups. Results: A total of 156 miRNAs in the aberrant miRNA profiles across the miRNA microarray showed differential expression (at least a 2-fold change, P ＜ 0.05) in cancer tissue, compared to noncancerous mucosa in both of H. pylori-negative and -positive samples. After 10 promising miRNAs were selected, validations by TaqMan miRNA assays confirmed that two miRNAs (hsa-miR-135b-5p and hsa-miR-196a-5p) were significantly increased and one miRNA (hsa-miR-145-5p) decreased in cancer tissue compared to non-cancerous gastric mucosa at H. pylori-negative group. For H. pylori-positive group, three miRNAs (hsa-miR-18a-5p, hsa-miR-135b-5p, and hsa-miR-196a-5p) were increased in cancer tissue. hsa-miR-135b-5p and hsa-miR-196a-5p were increased in gastric cancer in both of H. pylori-negative and -positive. Conclusions: miRNA expression of the gastric cancer implies that different but partially common gastric cancer carcinogenic mechanisms might exist according to H. pylori status.

참고문헌 (21)

참고문헌 신청

Jae Kwan Jun / 2017 / Effectiveness of the Korean National Cancer Screening Program in Reducing Gastric Cancer Mortality / Gastroenterology 152(6):1319~1328.e7

Belair C / 2009 / Helicobacter pylori and gastric cancer: possible role of microRNAs in this intimate relationship / Clin Microbiol Infect 15:806~812

Seiji Shiota / 2017 / Clinical Manifestations of Helicobacter pylori –Negative Gastritis / Clinical Gastroenterology and Hepatology 15(7):1037~1046.e3

Nordenstedt H / 2013 / Helicobacter pylori-negative gastritis: prevalence and risk factors / Am J Gastroenterol 108:65~71

Matsushima K / 2011 / MicroRNA signatures in Helicobacter pylori-infected gastric mucosa / Int J Cancer 128:361~370

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